两种分析食用植物油中抗氧化剂方法的比较

Comparison of Two Methods for Analysis of Antioxidants in Vegetable Oil

为了比较气相色谱/氢火焰离子化法(GC/FID)和高效液相色谱/二极管阵列法(HPLC/DAD)两种方法对植物油中BHA、BHT、TBHQ三种酚类抗氧化剂含量的检测。样品采用无水乙醇提取、冷冻离心后分别采用C18柱和HP-5毛细管柱进行分离,采用外标法进行定量测定。三种抗氧化剂在0~50 mg/L的浓度范围内线性关系良好。气相色谱法测定BHA、BHT、TBHQ三种成分的平均添加回收率在92.2%~95.1%之间,相对标准偏差在2.45%~3.08%之间,检出限分别为1 mg/kg、1 mg/kg和2 mg/kg。高效液相色谱法测定BHA、BHT、TBHQ三种成分的平均添加回收率在91.5%~94.3%之间,相对标准偏差在2.57%~2.92%之间,检出限分别为4 mg/kg、4 mg/kg和2 mg/kg。两种方法皆准确、灵敏、重现性好,适用于植物油中抗氧化剂的检测。

To compare the determination of BHA, BHT and TBHQ in vegetable oil by chromatography/hydrogen flame ionization detector(GC/FID) and high-performance liquid chromatography/photodiode array detector(HPLC/DAD), the samples were extracted with absolute ethanol, puritied by refrigerated centrifuge, separated by C18 column and HP-5 capillary column respectively, and quantified by external standard method. The results showed that 3 antioxidants had good linear relationship in the range of 0~50 mg/L. The average recoveries of antioxidants were between 92.2 %~95.1 %, RSD were between 2.45 %~3.08 %, the limits of detection were 1 mg/kg, 1 mg/kg and 2 mg/kg by GC, separately. The average recoveries of antioxidants were between 91.5 %~94.3 %, RSD were between2.57 %~2.92 %, the limits of detection were 4 mg/kg, 4 mg/kg and 2 mg/kg by HPLC, separately. Both methods were accurate, sensitive, highly reproducible and suitable for the analysis of antioxidants in vegetable oil.