牦牛GHR基因exon 10 SNP对乳成分影响的研究

Effect of Exon 10 SNP Polymorphism of GHR Gene on Yak Milk Composition

对牦牛GHR基因exon 10片段SNP多态性检测结果表明,相对于普通牛GHR exon 10(Genebank登录号AM161140.1),青海泽库高原型牦牛GHR基因exon 10含SNP多态位点8个,第267位G(GG/AG),第336位T(TT/GT),第650位A(AA/AC),第702位T(C/C型),第730位A(A/G型),第755位C(T/T型),第810位T(C/T型),第989位T(C/C型);SNP多态位点共形成4种基因型,GG/GT/AA/CC/AA/TT/CT/CC (基因型Ⅰ)1头、GG/TT/AC/CC/AG/TT/TT/CC(基因型Ⅱ) 5头、GG/TT/AA/CC/AA/TT/TT/CC 12头(基因型Ⅲ)、AG/TT/AA/CC/AA/TT/TT/CC (基因型Ⅳ)2头,各基因型占比为1∶5∶12∶2,其中GG/TT/AA/AA/TT为优势基因型。4种基因型间各乳成分差异显著性分析表明,牦牛GHR基因exon 10各SNP均为同义突变,未造成泌乳性状的改变。

GHR is considered as an important candidate gene for lactation traits.The study of GHR gene SNP polymorphism in yak is an important basis for the next step of performance marker-assisted breeding of yak milk.We tested the SNP polymorphic on exon 10 segments of GHR gene.The results showed that yak GHR gene exon 10 contains 8 SNP polymorphic loci,the 267th G(GG/AG),the 336th T(TT/GT),the 650th A(AA/AC),the 336th T(TT/GT),the 650th A(AA/AC),the 702th T(C/C),the 730th A(A/G),the 755th C(T/T),th 810th T(C/T)the 989th T(C/C);all SNP form four kinds of genotypes,1 GG/GT/AA/CC/AA/TT/CT/CC(Genetype Ⅰ)、five GG/TT/AC/CC/AG/TT/TT/CC(Genetype Ⅱ),12 GG/TT/AA/CC/AA/TT/TT/CC(GenetypeⅢ),2 AG/TT/AA/CC/AA/TT/TT/CC(Genetype Ⅳ),GenetypeⅢ for advantage.The results showed that all SNPS of THE GHR gene Exon 10 in yak were synonymous mutations,which did not change the lactation characteristics.