口蹄疫病毒受体整联蛋白在绵羊不同发育阶段组织中mRNA转录水平的分析

Quantitative Analysis of FMDV Receptor Integrin mRNA Levels in Ovine Tissues at Different Developmental Stages

口蹄疫病毒野毒株利用整联蛋白(integrin)αvβ1、αvβ3、αvβ6和αvβ8作为受体侵入细胞。本研究采用实时逆转录聚合酶链反应(qRT-PCR)技术,定量检测绵羊胎儿(45、75和110 d的绵羊胎儿)、新生羔羊和成年绵羊组织中整联蛋白亚单位αv、β3、β6和β8转录水平。比较分析发现,在蹄冠上皮组织中,成年绵羊和新生羔羊的β6 mRNA水平低于75和110 d胎儿的;在心组织中,成年绵羊、新生羔羊和110 d胎儿的β6 mRNA高于45和75 d胎儿的;在肌肉组织中,胎儿和新生羔羊的β6以及β8 mRNA水平高于成年绵羊的;在舌上皮组织中,成年绵羊的β3 mRNA水平低于其他发育时期的。在软腭组织、扁桃体中整联蛋白mRNA水平在胎儿、新生儿和成年绵羊之间变化不大。本研究首次获得相关整联蛋白mRNA在绵羊不同发育阶段组织中分布的定量数据,为了解整联蛋白在口蹄疫病毒感染组织嗜性中的作用机制提供新的科学依据。

Field strains of foot-and-mouth disease virus(FMDV)utilize integrin heterodimersαvβ1,αvβ3,αvβ6 andαvβ8 as receptors to gain entry to cells.Using novel real-time reverse transcriptase-polymerase chain reaction(RT-PCR)assays,levels of mRNA of the integrin subunitsαv,β3,β6 andβ8 were quantified in tissue samples taken from ovine foetuses at 45,75 and 110 days of gestation,neonatal lambs and adult ewes.The results showed that in the coronary band that the level ofβ6 mRNA of adult ewes and neonatal lambs was lower than that of foetuses at 75 and 110 days of gestation;In the heart,the level ofβ6 mRNA of adult ewes,neonatal lambs and foetuses at 110 days of gestation was higher than that of foetuses at 45 and 75 days of gestation;In the muscle,the level ofβ6 andβ8 mRNA of foetuses and neonatal lambs was higher than that of foetuses at 45 and 75 days of gestation.In the tongue epithelium,the level ofβ3 mRNA in adult ewes was lower than that in other stages.In the soft palate and tonsil,there was little variation in the level of integrin mRNA between stages.In this study,the quantitative changes in integrin subunit mRNA distribution in sheep across different developmental stages have been assessed for the first time,which would provide a useful insight into the role of integrins in the pathogenesis of FMD in vivo.