探讨磁珠法与煮沸法对乙型肝炎病毒基因分型检出率及其与HBV DNA定量的关系

Study the HBV genotype detection rate based on magnetic activated cell sorting and boiling method and its correlation with HBV DNA quantitation

目的:研究磁珠法与煮沸法用于检测乙型肝炎病毒(HBV)基因分型的检出率,并探讨基因分型检出情况与HBV DNA定量间的关系。方法:2019年6月-2019年9月收治乙肝患者95例,随机分为两组,A组应用磁珠法,B组应用煮沸法对血浆进行处理,提取HBV DNA后通过实时荧光聚合酶链式反应(PCR)进行肝炎基因分型及DNA定量。比较两组患者检测结果并探讨基因分型结果检出率与HBV DNA定量水平的关联。结果:A组患者B型、D型、B/C混合型及总检出率均显著高于B组,C型检出率显著低于B组,差异有统计学意义(P<0.05);两种方法检测HBV基因型检出率与HBV DNA水平呈正比,但应用磁珠法对低DNA水平样本的基因型检出明显优于煮沸法。结论:磁珠法提取核酸阳性率与灵敏度均较高,对于非C型及低病毒载量患者具有较高检出率。

Objective:To discuss the HBV(hepatitis B virus)genotype detection rate based on magnetic activated cell sorting and boiling method and explore the relationship between the detection of genotyping and HBV DNA quantification.Methods:From June 2019 to September 2019,95 patients with hepatitis B were selected,they were randomly divided into the two groups.The group A used magnetic activated cell sorting.The group B used boiling methodto process plasma and extract HBV DNA.HBV genotypes and HBV DNA quantification were measured through real-time fluorescent polymerase chain reaction(PCR).We compared the test results of the two groups of patients and explore the correlation between the detection rate of genotyping results and the quantitative level of HBV DNA.Results:The detection rate of type B,type D,B/C mixed type and total detection rate of the group A was significantly higher than that of the group B,and the detection rate of type C was significantly lower than that of the group B,the difference was statistically significant(P<0.05).HBV genotype detection rate based on two examination methods was positively correlated with HBV DNA levels.However,HBV genotype detection rate for low DNA samples based on magnetic activated cell sorting was significantly better than boiling method.Conclusion:The magnetic activated cell sorting can increase the positive acid rate and diagnosis sensitivity,especially for patients with non-C genotypes and low viral loads.