QuEChERS-气相色谱串联质谱法测定香菇中13种农药残留

Determination of 13 Pesticide Residuesin Lentinus edodes by QuEChERS-GC-MS/MS

建立基于QuEChERS-气相色谱串联质谱法(GC-MS/MS)快速检测香菇中13种农药残留的分析方法。香菇样品经前处理后,气相色谱分离,多反应离子监测模式(MRM)进行质谱检测,用外标法定量。结果表明,在0.01、0.10、0.50 mg/kg三个添加水平下,回收率范围为75.4%~110.6%。用重复性限反映精密度,均符合标准GB 23200.113—2018的要求。QuEChERS-气相色谱串联质谱法快速、高效、可靠,适用于香菇样品中的农药残留检验检测。

A rapid method for the determination of 13 pesticide residues in Lentinus edodes was established based on QuEChERS-gas chromatography-mass spectrometry(GC-MS/MS).After pretreatment,the Lentinus edodes samples were separated by gas chromatography,detected by mass spectrometry in the multi-reaction ion monitoring mode(MRM),and quantitatively analyzed with the external standard method.The results showed that the 13 pesticides had good linearity in the range from 0.01μg/mL to 0.8μg/mL,and the correlation coefficients R2>0.99.The average recovery ranges were mainly from 75.4%to 110.6%at the three spiked concentration levels(0.01、0.10、0.50 mg/kg).The repeatability limit is used to reflect the precision,which all were accepted the requirements of standard GB 23200.113—2018.The method is rapid,efficient and reliable,which is suitable for the detection of pesticide residues in Lentinus edodes samples.