摘要
用乙醇室温浸泡提取苦木,浓缩乙醇后得到提取物,提取物经酸溶碱沉后,用乙酸乙酯萃取碱水液,浓缩乙酸乙酯得到总生物碱。总碱通过硅胶柱层析分离纯化,得到一化合物,经核磁鉴定为苦木酮。对苦木中苦木酮的含量进行了测定,并对苦木酮进行了人源肠内菌转化研究,结果显示苦木酮在人源肠道微生物及微生物分泌的酶作用下发生结构变化,转化成活性增强的苦木碱丁。
The plant of Picrasma quassioides(D.Don)Benn.was extracted with ethanol.The concentrated extract was dissolved in aqueous hydrochloric acid solution,and then basified and partitioned with EtOAc to obtain crude alkaloids after ethyl acetate removal.The crude alkaloids was purified by silica gel column chromatography,and the compound was designated as 4-methoxy-5-hydroxycanthin-6-dione based on nuclear magnetic resonance(NMR).After biotransformation by human intestinal flora,4-methoxy-5-hydroxycanthin-6-dione was transformed into 4,5-dimethoxycanthin-6-one.
作者
陈封政
龙洁
孙国峰
田冲
李书华
CHEN Fengzheng;LONG Jie;SUN Guofeng;TIAN Chong;LI Shuhua(School of Chemistry,Resources and Environment,Leshan Sichuan 614000,Chinna;Leshan Engineering Research Center for Medicinal Components of Characteristic Agro-Products,Leshan Normal University,Leshan Sichuan 614000,Chinna;Soild-state Fermentation Resource Utilization Key Laboratory of Sichuan Province,Yibin Sichuan 644000,China)
出处
《乐山师范学院学报》
2021年第8期14-19,共6页
Journal of Leshan Normal University
基金
四川省科技厅资助项目“基于仿生法建立研究中药效应物质的新方法”(2018JY0065)
药食同源植物资源开发四川省高校重点实验室资助项目“大宗药材常山活性成分的分离与质量标准的建立”(10Y201801)
乐山师范学院资助项目“肠道微生物对中药成分的代谢及机理研究”(LZD001)
固态发酵资源利用四川省重点实验室资助项目“食品安全国家标准中红曲色素的研制”(2018GTJ011)。
关键词
苦木
人源肠内菌
生物转化
苦木酮
苦木碱丁
Picrasma Quassioides(D.Don)Benn.
Human Intestinal Flora
Biotransformation
4-methoxy-5-hydroxycanthin-6-dione
4,5-dimethoxycanthin-6-one
