陕西省草莓病毒种类的LncRNA测序初步鉴定

LncRNA Sequencing Identification of Viruses Infecting Strawberry in Shaanxi

为明确陕西省草莓主要病毒种类及各种病毒在主产区的地理分布,通过LncRNA测序结合反转录聚合酶链式反应(reverse transcription PCR,RT-PCR)对陕西省草莓主产区西安、咸阳和宝鸡的7个区或县的草莓疑似感染病毒病样品进行了检测。结果表明草莓斑驳病毒(strawberry mottle virus,SMoV)和草莓镶脉病毒(strawberry vein band virus,SVBV)在7个区或县均有发生,而草莓轻型黄边病毒(strawberry mild yellow edge virus,SMYEV)、菠菜潜隐病毒(spinach latent virus,SpLV)和番茄线虫传多面体病毒(Lycopersicon esculentum nepovirus,LENV)局部发生。首次报道在草莓上检测到Sp LV和LENV。

In order to clarify major virus species on strawberry and their geographical distribution in main strawberry areas in Shaanxi,this study collected and detected strawberry samples from seven districts or counties in Xi’an,Xianyang and Baoji through LncRNA sequencing and reverse transcription PCR(RT-PCR).Results showed that strawberry mottle virus(SmoV)and strawberry vein band virus(SVBV)were identified in all of the seven districts or counties,but strawberry mild yellow edge virus(SMYEV),spinach latent virus(SpLV)and Lycopersicon esculentum nepovirus(LENV)were only identified in parts of the seven districts or counties.SpLV and LENV were firstly identified in strawberry.