分析保护剂对烟草香味成分的基质效应补偿

Matrix effect compensation for analyte protectants during aroma component analysis of tobacco

为了解决目前强极性分析保护剂在弱极性溶剂体系中不适用的问题,以V_(乙腈)∶V_(二氯甲烷)=2∶1溶剂为弱极性溶剂体系,分别考察了23种化合物作为分析保护剂对31种香味成分色谱峰峰高和峰形的影响,并与烟草基质下各香味成分的色谱峰进行了对比。通过优化分析保护剂种类、组合及组合浓度,探索合适的分析保护剂组合,并另外选择15种烟草香味成分,对该分析保护剂组合的基质效应补偿效果进行验证。结果表明:①合适的分析保护剂组合为苹果酸+1,2-十四碳二醇(浓度均为1000 mg/L)。②在溶剂标准溶液中加入分析保护剂组合后,基质效应补偿效果良好,各香味成分标准曲线的线性得到了较大改善,其斜率及线性相关系数(r^(2))与基质匹配标准曲线基本一致。③加入分析保护剂可以补偿烟草香味成分GC-MS分析中存在的基质效应。

In order to solve the challenge that strong polar analyte protectants are not well performed in weak polar solvent systems,the solution of acetonitrile∶dichloromethane=2∶1(V/V)was chosen as weak polarity solvent system.The effects of 23 compounds as analyte protectants on the heights and shapes of chromatographic peaks of 31 aroma components were investigated.The chromatographic peaks of the aroma components in solvent added with these analyte protectants were compared with those in tobacco matrix.After optimizing the type,composition and concentration of the protectants,a suitable combination of analyte protectants was developed.In addition,15 aroma components in tobacco(other than the 31 aroma components mentioned above)were chosen to verify the matrix compensation effect of the developed analyte protectant combination.The results showed that:1)The suitable analyte protectants combination was malic acid(1000 mg/L)+1,2-tetradecanediol(1000 mg/L).2)By adding the optimized analyte protectants combination into the solvent standard solution,a satisfactory matrix compensation effect was achieved.The linearity of the calibration curve for each aroma component was greatly improved,and the slope and linear correlation coefficient(r^(2))were basically consistent with the matrix-matched calibration curve.3)The matrix effect of aroma components in tobacco during GC/MS analysis was compensated by adding analyte protectants.