风热清口服液对免疫低下小鼠免疫调节作用的研究

Study on Immunomodulatory Property of Fengreqing Oral Liquid on Immunosuppressive Mice

目的:探究风热清口服液对免疫低下小鼠免疫调节的作用。方法:通过给小鼠腹腔注射免疫抑制剂环磷酰胺50 mg/kg,1次/d,连续3 d,建立免疫功能低下的动物模型。应用碳廓清实验、溶血素生成实验和迟发型变态反应,评价风热清口服液对免疫低下小鼠非特异性免疫、体液免疫和细胞免疫的调节作用。结果:风热清口服液低、中剂量组可不同程度提高环磷酰胺致免疫低下小鼠的廓清指数K值(P<0.05)和吞噬指数α值(P<0.05)。风热清口服液低、中、高剂量组可不同程度提高免疫低下小鼠血清溶血素水平(P<0.05)。风热清口服液低、中剂量组可不同程度促进免疫低下小鼠迟发型变态反应(P<0.05)。风热清口服液低剂量组可提高免疫低下小鼠脾脏中CD4^(+)细胞数(P<0.01),并使CD4^(+)/CD8^(+)细胞比值上升(P<0.001),同时抑制免疫低下小鼠脾脏中CD8^(+)细胞数(P<0.05);风热清口服液中、高剂量组可提高免疫低下小鼠脾脏中CD4^(+)/CD8^(+)细胞比值(P<0.01),同时抑制免疫低下小鼠脾脏中CD8^(+)细胞数(P<0.05)。风热清口服液低、中剂量组可显著提高免疫低下小鼠脾指数(P<0.001),风热清口服液低、中、高剂量组均可提高免疫低下小鼠胸腺指数(P<0.05或P<0.01)。结论:风热清口服液通过提高免疫低下小鼠非特异性免疫、体液免疫和细胞免疫,进而增强免疫低下小鼠的免疫功能。

Objective:To study the immunomodulatory activity of Fengreqing Oral Liquid on immuno-suppressive mice.Methods:Through intraperitoneal injection of 50 mg/kg immunosuppressant cyclophosphamide into mice,once a day for 3 consecutive days,an animal model of immunocompromised function was established.Carbon clearance test,quantitative hemolysis test and DNFB induced delayed-type hypersensitivity(DTH)were applied to assay the regulating effects of Fengreqing Oral Liquid on nonspecific immunity,humoral immunity and cellular immunity in immunocompromised mice.Results:The clearance index K(P<0.05)and values of phagocytic indexα(P<0.05)of cyclophosphamide-induced immunocompromised mice could be elevated to varying degrees by Fengreqing Oral Liquid low and medium dose group.Productions of HClgM in serum were enhanced in different extent in immunosuppressive mice by Fengreqing Oral Liquid low,medium and high dose groups(P<0.05).Fengreqing Oral Liquid low and medium dose group variously increased DTH reactivity in immunosuppressive mice(P<0.05).Fengreqing Oral Liquid low dose group can not only increased percentages of T cells expressing CD4^(+)(P<0.01)in the spleen of immunocompromised mice,but also enhanced the ratio of CD4^(+)/CD8^(+)cells(P<0.001).And Fengreqing Oral Liquid low dose group suppressed percentages of T cells expressing CD8^(+)(P<0.05)in the spleen of immunocompromised mice.Fengreqing Oral Liquid medium and high dose group enhanced the ratio of the two subset of T lymphocyte(P<0.01),while suppressed percentages of T cells expressing CD8^(+)(P<0.05).Fengreqing Oral Liquid low and medium dose group obviously increased spleen index in immunosuppressive mice(P<0.001).Fengreqing Oral Liquid low,medium and high dose group all increased thymus index in immunosuppressive mice(P<0.05 or P<0.01).Conclusion:Fengreqing Oral Liquid enhances the immune function of immunocompromised mice by improving non-specific immunity,humoral immunity and cellular immunity of immunocompromised mice.