基于生物分子网络探讨桑白皮汤治疗慢性阻塞性肺疾病的作用机制

Mechanism of Sangbaipi Decoction in treatment of chronic obstructive pulmonary disease based on biological network

目的利用生物分子网络探讨桑白皮汤治疗慢性阻塞性肺疾病(COPD)的作用机制。方法通过中药系统药理数据库和分析平台(TCMSP)收集桑白皮汤的化学成分和成分对应的靶点,并利用Cytoscape软件构建中药-成分-靶点网络;通过GeneCards、TTD和DisGeNET数据库获取与COPD相关的靶点,对成分靶点和疾病靶点取交集,利用String和Cytoscape构建交集靶点网络;利用DAVID数据库对交集靶点进行KEGG和GO富集分析。结果筛选得到109个化学成分,230个成分靶点,COPD相关靶点1220个,成分和疾病的交集靶点119个。KEGG分析表明桑白皮汤可能通过PI3K-Akt信号通路、TNF信号通路、MAPK信号通路、NF-κB信号通路、HIF-1信号通路、凋亡相关通路等发挥治疗COPD的作用。GO分析显示桑白皮汤主要涉及炎症反应、细胞增殖、细胞凋亡、RNA转录、基因表达等。结论通过构建生物分子网络,揭示了桑白皮汤治疗COPD的分子机制,为桑白皮汤的后续研究提供了理论依据。

Objective To explore the mechanism of Sangbaipi Decoction in treatment of COPD through biological network.Methods The chemical components and corresponding targets of Sangbaipi Decoction were collected by TCMSP,and the network of herb-ingredient-target was constructed by Cytoscape software.The targets related to COPD were obtained from GeneCards,TTD,and DisGeNET databases.The intersection targets of components and disease were integrated.Target network of intersection targets was constructed using String online database and Cytoscape software.The results of KEGG and GO enrichment analysis of intersection targets was obtained from DAVID database.Results A total of 109 active components and 230 corresponding targets were obtained.Furthermore,1220 COPD related targets and 119 intersection targets were obtained.KEGG analysis revealed that Sangbaipi Decoction might treat COPD through PI3 K-Akt signaling pathway,TNF signaling pathway,MAPK signaling pathway,NF-κB signaling pathway,HIF-1 signaling pathway,and Apoptosis-related pathway.GO analysis demonstrated that Sangbaipi Decoction was mainly involved in inflammatory reaction,cell proliferation,apoptosis,RNA transcription,gene expression.Conclusion The molecular mechanism of Sangbaipi Decoction in treatment of COPD were discussed,which might provide a theoretical basis for further exploration.