SIRT3 HIF-1αFAP和HGF在不同临床分期胃癌组织中的表达及意义

Expression and significance of SIRT3,HIF-1α,FAP and HGF in gastric cancer tissues in different clinical stages

目的探讨去乙酰化酶3(SIRT3)、缺氧诱导因子-1α(HIF-1α)、成纤维细胞活化蛋白(FAP)和肝细胞生长因子(HGF)在不同临床分期胃癌组织中的表达及意义。方法选取2017年6月~2018年12月秦皇岛市第一医院收治的胃癌患者83例,取患者原发肿瘤组织为癌组织标本,同时选取相对应的癌旁正常组织标本为对照,采用免疫组化法检测癌组织和癌旁正常组织中SIRT3、HIF-1α、FAP和HGF的表达水平,分析SIRT3、HIF-1α、FAP和HGF表达与胃癌临床分期的关系及在胃癌组织中表达的相关性。结果SIRT3在胃癌组织中的阳性表达率低于癌旁组织(P<0.05),HIF-1α和HGF在胃癌组织中的阳性表达率高于癌旁组织(P<0.05),FAP在胃癌组织中的阳性表达率为51.81%,在癌旁组织中不表达(P<0.05);SIRT3、HIF-1α、FAP和HGF在胃癌组织中的表达与胃癌TNM分期显著相关(P<0.05);胃癌组织中SIRT3与HIF-1α、FAP、HGF的表达呈负相关(P<0.05);HIF-1α与FAP、HGF的表达呈正相关(P<0.05);FAP与HGF的表达呈正相关(P<0.05)。结论SIRT3、HIF-1α、FAP和HGF的表达水平与胃癌的TNM分期存在相关性,它们可能共同参与了胃癌的发生发展,SIRT3的表达可能起到负向调控HIF-1α、FAP和HGF的作用。

Objective To investigate the expression and significance of Sirtuin 3(SIRT3),hypoxia-inducible factor-1α(HIF-1α),fibroblast activation protein(FAP)and hepatocyte growth factor(HGF)in gastric cancer tissues in different clinical stages.Methods Cancer tissue samples were collected from 83 patients with gastric cancer,and corresponding adjacent normal tissue samples were selected as control.Immunohistochemical methods were used to detect the expression levels of SIRT3,HIF-1α,FAP and HGF in cancer tissues and normal tissues adjacent to the cancer.The relationship between the expression of SIRT3,HIF-1α,FAP and HGF and the clinical stage of gastric cancer and the correlation in gastric cancer tissue were analyzed.Results The positive expression rate of SIRT3 in gastric cancer tissues was lower than that in adjacent tissues(P<0.05),but the positive expression rates of HIF-1αand HGF in gastric cancer tissues were higher than those in adjacent tissues(P<0.05).The positive expression rate of FAP in gastric cancer tissues was 51.81%,which was not expressed in adjacent tissues(P<0.05).The expressions of SIRT3,HIF-1α,FAP and HGF in gastric cancer tissues were significantly correlated with TNM stage of gastric cancer(P<0.05).SIRT3 was negatively correlated with the expression of HIF-1α,FAP and HGF in gastric cancer tissues(P<0.05),HIF-1αwas positively correlated with the expression of FAP and HGF(P<0.05),and FAP was positively correlated with the expression of HGF(P<0.05).Conclusion The expression levels of SIRT3,HIF-1α,FAP and HGF are correlated with the TNM stage of gastric cancer.They may jointly participate in the occurrence and development of gastric cancer,and the expression of SIRT3 may play a negative role in regulating HIF-1α,FAP and HGF.