具核梭杆菌感染的巨噬细胞对人舌鳞癌细胞系Cal-27细胞增殖与迁移的影响

Effects of macrophages induced by Fusobacterium nucleatum on proliferation and migration of tongue squamous cell carcinoma Cal-27 cells

目的:观察具核梭杆菌(F.nucleatum)诱导的巨噬细胞对人舌鳞癌细胞系Cal-27细胞增殖、迁移的影响。方法:F.nucleatum以感染复数(MOI)100∶1,刺激THP-1细胞来源的巨噬细胞,24 h后收集细胞条件培养液,以THP-1细胞及THP-1细胞来源巨噬细胞的条件培养液为对照。将获得的各组条件培养液与Cal-27细胞共培养,CCK-8法检测各组细胞增殖水平的变化,划痕实验检测Cal-27细胞迁移能力的变化。ELISA检测各组条件培养液中肿瘤坏死因子α(TNF-α)及白细胞介素(IL)-6表达水平。结果:与2个对照组相比,F.nucleatum诱导的巨噬细胞培养液上清使Cal-27细胞增殖减少(P<0.05),相对迁移率增高(P<0.05)。F.nucleatum刺激巨噬细胞后,条件培养液中TNF-α和IL-6水平均较刺激前明显增加(P<0.05)。结论:F.nucleatum感染能促进巨噬细胞分泌TNF-α和IL-6,进而可能促进Cal-27细胞迁移,抑制其增殖。

Objective:To observe the effects of infection induced by Fusobacterium nucleatum(F.nucleatum)on the proliferation and infiltration of tongue squamous cell carcinoma cell line Cal-27.Methods:Supernatant from THP-1 cells differentiated macrophages stimulated with F.nucleatum(multiplicity of infection was 100∶1)for 24 h was collected,and supernatants from unstimulated THP-1 cells differentiated macrophages and THP-1 cells were collected as controls.Cal-27 cells were co-cultured with the above-mentioned supernatants and Cal-27 cells proliferation was detected by CCK-8 method.Migration of Cal-27 cells were evaluated by scratch assay.Expressions of tumor necrosis factor-α(TNF-α)and interleukin(IL)-6 were detected by ELISA.Results:F.nucleatum-induced macrophages conditioned medium reduced the proliferation but promoted the migration rate of Cal-27 cells in comparison with two control groups(P<0.05).The levels of TNF-αand IL-6 in macrophages stimulated with F.nucleatum were significantly higher than those without any stimulations(P<0.05).Conclusions:Stimulation of macrophages with F.nucleatum promoted TNF-αand IL-6 production,which may promote the migration but inhibit the proliferation of Cal-27 cells.