基于转录组RNA测序分析牙髓炎中的核心基因

Analysis of hub genes in pulpitis based on transcriptome RNA sequencing

目的:筛选炎症牙髓与健康牙髓的差异表达基因。方法:对5例炎症牙髓样本和6例健康牙髓样本进行转录组RNA测序,筛选差异表达基因,进行生物信息学分析。结果:一共筛选出1216个差异表达基因,包括831个上调基因和385个下调基因。GO分析结果显示,差异基因主要富集于炎症反应、免疫反应和细胞粘附等功能;KEGG通路分析表明,差异基因主要富集于细胞因子-细胞因子受体相互作用、趋化因子信号通路、破骨细胞分化等炎症相关通路。基因集富集分析结果也富集于炎症反应通路、TNF-α信号通路以及IL6/JAK/STAT3信号通路。根据PPI网络中的节点连通度,共筛选出10个与牙髓炎相关的核心基因:PTPRC、ITGAM、CD86、IL10、TLR4、ITGB2、TYROBP、SPI1、LCP2、TLR2。结论:炎症牙髓与健康牙髓在整体基因表达上存在较显著的差异,差异表达基因大多富集于炎症和免疫反应的功能及信号通路,相关基因具有作为牙髓炎诊断生物标志物的潜在价值。

Objective:Screening for differentially expressed genes between inflammatory pulp and healthy pulp.Methods:Transcriptome RNA sequencing was performed on 5 inflammatory dental pulp samples and 6 healthy dental pulp samples.Differentially expressed genes were screened for bioinformatics analysis.Results:A total of 1216 differentially expressed genes were screened out,including 831 up-regulated genes and 385 down-regulated genes.GO analysis revealed that the differentially expressed genes were concentrated in the functional sets consisting of inflammatory response,immune response and cell adhesion.KEGG pathway analysis showed that the differentially expressed genes were enriched in inflammatory pathways such as cytokine-cytokine receptor interactions,chemokine signaling pathways and osteoclast differentiation.GSEA results were also enriched in the inflammatory response pathway,TNF-αsignaling pathway,and IL6/JAK/STAT3 signaling pathway.According to the degrees of nodes in the PPI network,10 hub genes related to pulpitis were screened out:PTPRC,ITGAM,CD86,IL10,TLR4,ITGB2,TYROBP,SPI1,LCP2,TLR2.Conclusions:There were significantly statistical differences in the overall gene expression between inflammatory and healthy pulp.Most of the differentially expressed genes are concentrated in the functions and signaling pathways of inflammation and immune response,and the related genes have potential value as biomarkers for the diagnosis of pulpitis.