联合检测miRNA129与调节性T细胞对诊断结肠癌的临床意义

Clinical Significance of Combined Detection of miRNA129 and Regulatory T Cell in Screening Colon Cancer

目的探讨联合检测微小核糖核酸miRNA129与调节性T细胞(Treg)对诊断结肠癌的价值.方法选取62例结肠癌患者为研究组,62例健康者为对照组,应用荧光定量PCR法检测miRNA129在两组患者中的相对表达量,同时应用流式细胞仪检测外周血中Treg细胞的百分数,评估miRNA129与Treg联合检测对诊断结肠癌的价值.结果结肠癌组血清中miRNA 129的相对表达量高于健康组,差异具有统计学意义(P<0.05).同时,结肠癌组血清中Treg细胞百分数显著高于健康组,差异具有统计学意义(P<0.05).结论miRNA129与Treg细胞在结肠癌患者血清中呈现高表达状态,miRNA129与Treg细胞可能成为结肠癌早期诊断的参考指标.

Objective To explore the clinical significance of combined detection of miRNA129 and regulatory T cell(Treg)in screening colon cancer.Method62 colon cancer patients were selected in the study group and 62 healthy patients in the control group.Fluorescence quantitative PCR method was used to detect the relative expression of miRNA129 in two groups.The clinical significance of miRNA129 combined with Treg in screening colorectal cancer was evaluated by using percentage of Treg in peripheral blood by flow cytometry.ResultsThe relative expression of serum miRNA 129 in colon cancer group was higher than that in healthy group(P<0.05).Meanwhile,the percentage Treg in colon cancer group was significantly higher than that in healthy group(P<0.05).ConclusionmiRNA129 and Treg cell present a high expression state in the serum of colon cancer patients,and the combined detection of miRNA129 and Treg cells has important value in screening colon cancer,which may become a reference index for early screening of colon cancer.