CARD9 PKM2 CMTM6在乳腺癌组织中的表达及相关性研究

Expression and Correlation of CARD9 PKM2 and CMTM6 in Breast Cancer Tissues

目的:探究胱天蛋白酶募集域蛋白9(CARD9)、M2型丙酮酸激酶(PKM2)、趋化因子样因子超家族成员6(CMTM6)在乳腺癌组织中的表达及相关性。方法:选取2019年11月至2020年11月我院接诊的79例乳腺癌患者研究对象。免疫组化法检测癌组织、癌旁组织中CARD9、PKM2、CMTM6表达情况,实时荧光定量(RT-PCR)检测癌组织、癌旁组织中CARD9、PKM2、CMTM6表达情况。分析CARD9、PKM2、CMTM6表达与乳腺癌的关系。结果:与癌旁组织相比,癌组织中CARD9、PKM2、CMTM6表达较高(P<0.05)。与高中分化程度、无淋巴结转移、Ⅰ~Ⅱ期乳腺癌患者相比,低分化程度、有淋巴结转移、Ⅲ~Ⅳ期乳腺癌患者CARD9、PKM2、CMTM6表达较高(P<0.05)。CARD9、PKM2、CMTM6之间相关性分析显示CARD9、PKM2之间正相关(r=0.257,P=0.022);CARD9、CMTM6之间正相关(r=0.260,P=0.021);PKM2、CMTM6之间正相关(r=0.254,P=0.024)。与CARD9、PKM2、CMTM6单项相比,三者联合对乳腺癌的预测价值较高,具有统计学差异(P<0.05)。结论:CARD9、PKM2、CMTM6在乳腺癌组织中呈异常高表达且CARD9、PKM2、CMTM6呈线性正相关,与患者分化程度、淋巴结转移、临床分期相关,参与乳腺癌的发生、发展过程,三项联合检测对乳腺癌的预测价值较高。

Objective:To investigate the expression and correlation of caspase recruitment domain protein 9(CARD9),pyruvate kinase M2(PKM2),and chemokine-like superfamily member 6(CMTM6)in breast cancer tissues.Methods:A total of 79 patients with breast cancer admitted to our hospital from November 2019 to November 2020 were selected as research objects.The expressions of CARD9,PKM2 and CMTM6 in cancer tissues and paracancerous tissues were detected by immunohistochemistry,and the expressions of CARD9,PKM2 and CMTM6 in cancer tissues and paracancerous tissues were detected by real-time fluorescence quantitative(RT-PCR).To analyze the relationship between CARD9,PKM2,CMTM6 expression and breast cancer.Results:Compared with paracancerous tissues,CARD9,PKM2 and CMTM6 expressions were higher in cancer tissues(P<0.05).With high degree of differentiation,lymph node metastasis,ⅠⅡperiod compared to patients with breast cancer,low degree of differentiation,lymph node metastasis,Ⅲperiod breast cancer patients CARD9Ⅳ,PKM2,CMTM6 had higher expression among patients of low degree of differentiation,lymph node metastasis andⅢ-Ⅳperiod than patients of mediun-high degree of differentiation,no lymph node metastasis and I-II period(P<0.05).Correlation analysis among CARD9,PKM2 and CMTM6 showed a positive correlation between CARD9 and PKM2(r=0.257,P=0.022).Card9 and CMTM6 were positively correlated(r=0.260,P=0.021).PKM2 and CMTM6 were positively correlated(r=0.254,P=0.024).Compared with CARD9,PKM2 and CMTM6,the combination of these three items had higher predictive value for breast cancer,with statistical difference(P<0.05).Conclusion:The facts that CARD9,PKM2,CMTM6 have abnormally high expression in breast cancer tissue and CARD9,PKM2,CMTM6 are linearly positively correlated are related with the degree of differentiation,lymph node metastasis,clinical stages,and participation in the occurrence and development of breast cancer.The three combined tests have high predictive value for breast cancer.