可德兰多糖-阿魏酸接枝共聚物的制备工艺优化

Synthetic Optimization of Ferulic Acid/Curdlan Graft Copolymers

采用自由基介导的接枝方法制备可德兰多糖-阿魏酸(FA)接枝共聚物,通过单因素试验考察FA添加量、维生素C(Vc)添加量、过氧化氢(H_(2)O_(2))浓度和反应时间对接枝率和得率的影响,在此基础上,利用正交试验设计进一步优化接枝共聚物的制备工艺参数。研究表明,可德兰多糖-阿魏酸接枝共聚物制备的最佳工艺条件为:25 mL 5 mg/mL可德兰多糖溶液,FA和Vc添加量分别为250 mg和50 mg,H_(2)O_(2)浓度5 mol/L,反应时间12 h,此时,可德兰多糖-FA接枝共聚物的接枝率和得率均最大,分别为98.62 mg FA/g和40.84%,并通过紫外和红外光谱验证了接枝反应的发生。因此,该方法不仅改善了可德兰多糖的理化特性,而且能够提高可德兰多糖-FA接枝共聚物的接枝率,为其在食品工业中的应用奠定了理论基础。

A free-radical-mediated grafting method was used to prepare ferulic acid(FA)/curdlan graft copolymers.The effects of FA and vitamin C(Vc)addition,hydrogen peroxide(H_(2)O_(2))concentration,and reaction time on the grafting ratio and yield of FA/curdlan graft copolymers were investigated using single factor experiments.Accordingly,the synthetic parameters of FA/curdlan graft copolymers were further optimized through orthogonal experiments.The results demonstrated that the optimal process conditions for the preparation of FA/curdlan graft copolymers included 25 mL of 5 mg/mL curdlan solution,250 mg of FA,50 mg of Vc,5 mol/L H_(2)O_(2),and a reaction time of 12 h.Under these conditions,the highest grafting rate and yield of the FA/curdlan graft copolymers of 98.62 mg FA/g and 40.84%,respectively,were obtained.Grafting was also confirmed by ultraviolet and infrared spectroscopy analyses.Therefore,the proposed preparation technique not only improved the physicochemical properties of curdlan,but also increased the grafting ratio of the graft copolymers.The findings of this study provide a theoretical foundation for the practical applications of curdlan in food industry.