年龄对水合氯醛诱导的小鼠可逆性晶状体混浊及Na^(+)-K^(+)-ATP酶表达的影响

Effect of age on reversible lens opacity and Na^(+)-K^(+)-ATPase expression induced by chloral hydrate in mice

目的:探究年龄对水合氯醛诱导的小鼠急性可逆性晶状体混浊及Na^(+)-K^(+)-ATP酶表达的影响。方法:青年(3月龄)和老年(24月龄)C57BL/6小鼠各12只,4%水合氯醛(400mg/kg)腹腔注射诱导急性可逆性晶状体混浊。在注射后10、20、30、45、60、90、120、150min分别应用裂隙灯观察晶状体混浊程度,根据晶状体混浊评价系统记录混浊程度分级情况并拍照。苏木素-伊红(HE)染色观察晶状体病理改变,免疫组织化学染色检测晶状体Na^(+)-K^(+)-ATP酶表达。结果:水合氯醛注射后两组小鼠晶状体混浊和消退过程相似,但青年组小鼠晶状体混浊出现早、持续时间略长,混浊厚重,呈乳白色;老年组小鼠晶状体混浊出现晚,持续时间略短,混浊轻薄,呈薄雾状。HE染色显示水合氯醛注射后晶状体上皮细胞(LECs)下皮质聚集大量水泡,浅层晶状体纤维细胞结构紊乱。免疫组织化学染色显示LECs及纤维Na^(+)-K^(+)-ATP酶的表达呈现阳性,水合氯醛注射前青年组和老年组小鼠LECs中Na^(+)-K^(+)-ATP酶表达较弱,注射后45min LECs中Na^(+)-K^(+)-ATP酶的表达上调,且老年组小鼠LECs的Na^(+)-K^(+)-ATP酶上调更为显著。结论:年龄影响水合氯醛诱导小鼠急性可逆性晶状体混浊,Na^(+)-K^(+)-ATP酶参与水合氯醛诱导的晶状体混浊。

AIM:To investigate the effect of age on the expression of Na^(+)-K^(+)-ATPase and acute reversible lens opacification induced by chloral hydrate in mice.METHODS:Acute reversible lens opacification was induced by intraperitoneal injection of 4%chloral hydrate(400mg/kg)in 3-month-old(young group)and 24-month-old(old group)C57BL/6 mice.The lens opacification was graded at 10,20,30,45,60,90,120 and 150min after chloral hydrate injection.The histopathological changes of lens were observed by hematoxylin eosin staining,and the expression of Na^(+)-K^(+)-ATPase in lens was detected by immunohistochemistry.RESULTS:The development of lens opacity is similar in young and old mice after chloral hydrate injection.The lens opacification in the young group appeared earlier,thicker and lasted longer than the old group.HE staining showed that many vesicles appeared in the cortex below lens epithelial cells(LECs),and the structure of superficial lens fiber cells were disordered after chloral hydrate injection.Immunohistochemical staining showed that the expression of Na^(+)-K^(+)-ATPase was positive in LECs and fibers.The expression of Na^(+)-K^(+)-ATPase in LECs were weak before chloral hydrate injection and up-regulated 45min after chloral hydrate injection in young and old groups.The up-regulation of Na^(+)-K^(+)-ATPase was stronger in the old group than in the young group.CONCLUSION:Age may play a role in the acute reversible lens opacification induced by chloral hydrate in mice.The expression of Na^(+)-K^(+)-ATPase is involved in lens opacity induced by chloral hydrate.