基于功能性油脂的川陈皮素纳米乳液制备及消化特性研究

Preparation and digestive characteristics of nobiletin nanoemulsions based on functional oils

该研究采用高能量法成功制备了基于功能性油脂(紫苏籽油、火麻仁油和山茶油)的川陈皮素纳米乳液,并通过体外模拟消化实验,系统对比了其在口腔、胃、小肠不同消化阶段的粒径分布、电位变化等关键参数,进而分析了其消化特性,研究了不同功能性油脂的消化规律以及对川陈皮素生物可及性的影响。结果表明,3种纳米乳液在消化过程中具有相似的粒径分布和电位变化,但消化程度具有显著差异,其中消化程度最大的是山茶油[(93.7±1.4)%],其次为火麻仁油[(83.0±1.8)%],最小的为紫苏籽油[(76.4±0.9)%]。同时,山茶油纳米乳液递送的川陈皮素生物可及性为(66.4±2.9)%,紫苏籽油纳米乳液为(48.0±1.5)%,火麻仁油纳米乳液为(46.0±1.0)%,表明富含单不饱和脂肪酸的山茶油纳米乳液最能提高川陈皮素的生物可及性。该研究为通过提高川陈皮素的生物可及性促进其产业化应用提供了一种新的途径。

In this study,nobiletin nanoemulsions based on functional oils(perilla seed oil,hemp oil,and camellia oil)were successfully prepared by a high-energy method.Then,their digestive properties in the oral cavity,stomach and small intestine,including key parameters such as particle size distribution and potential changes,the digestion laws of different oil phase and the effects on the bioaccessibility of nobiletin were systematically analyzed through in vitro digestion simulation experiments.The results indicated that the three prepared nanoemulsions had similar particle size distributions and potential changes during digestion,however,the degree of oil phase digestion was significantly different.Particularly,The highest degree of digestion was camellia oil[(93.7±1.4)%],followed by hemp oil[(83.0±1.8)%],and the minimum was perilla seed oil[(76.4±0.9)%].In terms of the effect on the bioaccessibility,the bioaccessibility of nobiletin in camellia oil nanoemulsion,perilla seed oil nanoemulsion and hemp seed oil nanoemulsion were(66.4±2.9)%,(48.0±1.5)%,and(46.0±1.0)%,respectively.These results suggested the superior effect in increasing the bioaccessibility of nobiletin by camellia oil nanoemulsion that rich in monounsaturated fatty.In conclusion,this study provides a new strategy to improve the bioaccessibility of nobiletin,and promote its industrial application.