花生全基因组抗病基因鉴定及其对青枯菌侵染的响应分析

Genome-wide identification of peanut resistance genes and their response to Ralstonia solanacearum infection

花生是主要的油料作物之一,在生产过程中受到多种病原微生物的危害。培育和选用抗病品种是防治病害最经济有效的途径之一,而抗病基因是植物抵御病原微生物的重要基因。本文首次对花生抗病基因进行全基因组鉴定,发掘抗病候选基因4156个,其中RLK、RLP、NL、CNL、TNL这5种典型抗病基因分别有536、490、232、182和149个。抗病基因在染色体上分布不均匀,多数抗病基因集中在B02染色体上。转录组测序发现,抗病材料中特异表达的基因有111个,感病材料中特异表达的基因有104个,抗、感病材料均有表达的基因2216个、均不表达的有1725个。筛选出第1类响应青枯菌诱导的抗病基因5个,第2类持续上调表达抗青枯病基因65个。qRT-PCR成功验证了1个抗病候选基因Arahy.5D95TJ。本文对花生抗病基因的鉴定分析,为后续研究抗病基因功能与花生抗病的分子育种提供重要参考。

Peanut is one of the main oil crops,which is harmed by many pathogenic microorganisms during growth and develop-ment period.Breeding and selection of disease-resistant varieties is one of the most economical and effective ways to control disease,and disease resistance genes are important genes for plant resistance to pathogenic microorganisms.Here,the whole genome-wide identification of peanut disease resistance genes was carried out for the first time.A total of 4156 candidate disease resistance genes were identified.Among them,536,490,232,182,and 149 genes were RLK,RLP,NL,CNL,and TNL,respec-tively.The distribution of disease resistance genes was uneven on chromosomes,and most of them were concentrated on chromo-some B02.Transcriptome profiling revealed that 111 genes were specifically expressed in resistant materials,104 genes were specifically expressed in susceptible materials,2216 genes were expressed in both resistant and susceptible materials,while 1725 genes were not expressed in both resistant and susceptible materials.Two kinds of differentiate expressed R genes were identified,including five genes in the first group responded to the infection of Ralstonia solanacearum at specific time and 65 genes in the second group which exhibited higher expressions in resistant cultivar than susceptible cultivar.A candidate gene Arahy.5D95TJ was successfully validated by qRT-PCR.In this study,the identification and analysis of peanut disease resistance genes provides the important reference for further research of their functions and molecular breeding of peanut disease resistance.