马铃薯GAUT基因家族的全基因组鉴定及表达分析

Genome-wide identification and expression analysis of potato GAUT gene family

半乳糖醛酸转移酶(GAUT)是一种参与催化糖基化反应的酶类,在植物生长发育过程中发挥着重要作用。本研究鉴定了马铃薯GAUT家族成员,并对其理化性质、染色体定位、基因结构、保守蛋白结构域、基因重复事件和表达模式进行了分析。结果表明,鉴定到的41个GAUT家族成员(StGAUT),不均匀的分布在10条染色体上。根据基因的结构和系统发育蛋白特征,将41个StGAUT分为4个亚组。共线性分析表明,StGAUT基因家族存在12对片段重复基因,均在纯化选择下进化。通过对马铃薯双单倍体(DM)的不同组织部位和非生物胁迫下的RNA-seq数据进行分析,筛选出了组织特异性表达及响应非生物胁迫的StGAUT基因。此外,进一步对不同四倍体栽培种彩色马铃薯的薯皮和薯肉进行RNA-seq测序和分析,获得了可能参与花色素苷生物合成的StGAUT基因。本研究结果为进一步阐明StGAUT基因在马铃薯中的功能提供了有价值的信息。

Galacturonyltransferases(GAUTs)are enzymes responsible for catalyzing glycosylation reactions and play an impor-tant role in the growth and development of plant.In this study,GAUT genes family members in potato(StGAUT)were identified,and their physical and chemical characteristics,distribution on chromosomes,gene structure,conserved motifs,gene duplication events,and expression patterns were analyzed.The results showed that a total of 41 StGAUTs were identified and distributed ex-tensively and unevenly on 10 chromosomes.According to their structural and phylogenetic protein features,these 41 StGAUT genes were divided into four subclasses.Collinearity analysis indicated that 12 pairs of StGAUTs were segmental duplication genes,and these gene pairs evolved under purifying selection.RNA-seq data of different tissues and abiotic stresses were used to analyze tissue-specific and abiotic stress-responses of the StGAUT genes in doubled monoploid potato(DM).Results revealed that StGAUT genes might be involved in anthocyanin biosynthesis in three different-colored potato cultivars based on RNA-seq data.The results provide valuable information regarding further functional elucidation of StGAUT genes in potato.