摘要
目的研究D-缬氨酸对巨噬细胞M1极化的影响。方法在不同浓度的D-缬氨酸情况下,通过CCK-8方法测定分析细胞毒性,利用实时PCR分析多个基因的mRNA表达。然后通过Elisa方法检测IL-6,IL-1β,TNF-α的蛋白表达,使用荧光显微镜分析iNOS表达。结果50mM D-缬氨酸对巨噬细胞没有细胞毒性,D-缬氨酸在mRNA和蛋白质水平下调IL-6,IL-1β和TNF-α的表达,细胞中iNOS也下调明显。结论D-缬氨酸抑制RAW264.7巨噬细胞中LPS/IFN-γ诱导的M1巨噬细胞极化。
Objective To study the effect of D-valine on the M1 polarization of macrophages.Methods In the case of different concentrations of D-valine,the cytotoxicity was analyzed by the CCK-8 method.The mRNA expression of multiple genes was analyzed by real-time PCR.Then the protein expression of IL-6,IL-1β,and TNF-αwas detected by Elisa method,and the expression of iNOS was observed by fluorescence microscope.Results 50 mM D-valine has no cytotoxicity to macrophages.D-valine down-regulates the expression of IL-6,IL-1βand TNF-αat mRNA and protein levels,and iNOS in cells is also down-regulated significantly.Conclusion D-valine inhibits the polarization of M1 macrophages induced by LPS/IFN-γin RAW264.7 macrophages.
作者
倪宇昕
王勇
邓艳芳
NI Yu-xin;WANG Yong;DENG Yan-fang(Department of Stomatology,Huazhong University of Science and Technology Union Shenzhen Hospital,Shenzhen 518052,China)
出处
《中国实验诊断学》
2021年第8期1221-1224,共4页
Chinese Journal of Laboratory Diagnosis
基金
深圳市南山区卫生科技计划(2020012)
华中科技大学协和深圳医院院内课题(2020021)。
