血清miR-223、HMGB1与乙肝患者HBV-DNA载量和肝功能的关系研究

Correlation of miR-223 and HMGB1 in serum with HBV-DNA titer and liver function among patients with hepatitis B

目的探讨慢性乙型肝炎(chronic hepatitis B,CHB)患者微小RNA-223(microRNA-223,miR-223)、高迁移率族蛋白B1(high mobility group box-1 protein,HMGB1)表达水平及其与乙型肝炎病毒(hepatitis B virus,HBV)DNA载量及肝功能的关系。方法选取本院收治的86例CHB患者为CHB组和78例健康体检者为对照组。测定HBV-DNA载量并分析CHB患者miR-223、HMGB1与肝功能指标的相关性及影响CHB发生的因素。结果CHB组miR-223、HMGB1、丙氨酸转氨酶(ALT)、天冬氨酸转氨酶(AST)、谷氨酰转移酶(GGT)、总胆红素(TBIL)高于对照组(P<0.05)。HBV-DNA高载量组miR-223、HMGB1高于HBV-DNA中载量组、HBV-DNA低载量组(P<0.05)。CHB患者miR-223与HMGB1呈正相关(P<0.05),二者与ALT、AST、GGT、TBIL均呈正相关(P<0.05)。miR-223、HMGB1、HBV-DNA载量是影响CHB发生的独立危险因素(P<0.05)。结论miR-223、HMGB1可一定程度反映CHB患者HBV-DNA载量及肝功能。

Objective To investigate the expression microRNA-223(miR-223)and high mobility group box-1 protein(HMGB1)in patients with chronic hepatitis B(CHB)and their relationship with hepatitis B virus(HBV)-DNA titer and liver function.Methods Eighty-six CHB patients in our hospital were selected as CHB group and 78 healthy persons as control group.The HBV-DNA titers were measured and the correlation between miR-223,HMGB1 and liver function indexes as well as the influencing factors of CHB were analyzed.Results The levels of miR-223,HMGB1,ALT,AST,GGT and TBIL in CHB group were higher than those in control group(P<0.05).The levels of miR-223 and HMGB1 in high HBV-DNA titer group were higher than those in medium HBV-DNA titer group and low HBV-DNA titer group(P<0.05).In CHB patients,miR-223 was positively correlated with HMGB1(P<0.05),and both were positively correlated with ALT,AST,GGT and TBIL(P<0.05).miR-223,HMGB1 and HBV-DNA titer were independent risk factors of CHB(P<0.05).Conclusions In CHB patients,miR-223 and HMGB1 can reflect HBV-DNA titer and liver function to a certain extent.