摘要
为探究云南分心木提取物对高尿酸血症(HUA)小鼠模型的治疗作用,以联合氧嗪酸钾及腺嘌呤灌胃给药复制高尿酸血症小鼠为模型,对云南分心木水提物、醇提物及两种去除无机元素提取物的治疗作用进行考察。经过14天连续给药后,检测血清尿酸(UA)、肌酐(Cr)、尿素氮(BUN)的含量、肝脏组织黄嘌呤氧化酶(XOD)的活力、肾脏组织中IL-1β和TNF-α的水平,观察肾组织病理结构变化,评价不同提取物对HUA小鼠模型的治疗作用。结果显示,与模型组相比,云南分心木4种样品不同程度的降低UA、BUN、Cr的含量,降低XOD的活性,下调IL-1β及TNF-α的水平,减轻病理结构变化,且醇提物的效果最为显著。表明云南分心木提取物,可通过调节肾功能、抑制XOD活性以及抗炎的作用,对HUA小鼠模型产生治疗作用。
The objective of this paper is to investigate the therapeutic effects of extract of Yunnan Diaphragma Juglandis Fructus(YN-DJF)on hyperuricemia(HUA)models in mice.The HUA models in mice were established by i.g.oxygen oxazine acid potassium and adenine,and the therapeutic effect of 4 YN-DJF extracts,including water extract,alcohol extract,and the water extracts with two different deionization treatments,were evaluated.After 14 days of continuous administration,the contents of UA,Cr,and BUN in serum,the XOD activity of liver tissue,and the level of IL-1βand TNF-αin kidney were tested,and the pathological structure changes of renal tissue were observed.The results showed that,compared with the model groups,4 YN-DJF extracts performed different therapeutic levels by reducing the content of UA,Cr,and BUN,suppressing XOD activity,reducing IL-1βand TNF-αlevel,and alleviating pathological structural changes.Alcohol extract of YN-DJF was the most effective among all extracts.It proved that extracts of YN-DJF could be applied to treat HUA mouse by improving renal function,suppressing XOD activity,and anti-inflammatory effects.
作者
张伟
陈雪洁
刘蓉
沈磊
田新雁
姜北
ZHANG Wei;CHEN Xue-jie;LIU Rong;SHEN Lei;TIAN Xin-yan;JIANG Bei(Institute of Materia Medica of Dali University,Dali University;Basic Medical College of Dali University,Dali University,Dali 671000,China)
出处
《天然产物研究与开发》
CAS
CSCD
2021年第9期1463-1469,共7页
Natural Product Research and Development
基金
国家自然科学基金(81760646)。
关键词
云南分心木
提取物
高尿酸血症
肾功能
Diaphragma Juglandis Fructus of Yunnan
extracts
hyperuricemia
renal function