阿托品的优化给药方案对豚鼠近视治疗作用的研究

Study on the effect of optimized dosing regimen of atropine on the treatment of myopia in guinea pigs

目的研究优化的阿托品给药方案对豚鼠近视的治疗作用。方法从46只21 d龄豚鼠中随机选取6只作为空白对照,其余40只随机分为5个干预组:1%阿托品组、0.01%阿托品组、优化组1、优化组2及生理盐水组。随机选择干预组豚鼠单眼作为模型眼并予形觉剥夺,对侧眼为自身对照,干预时间均为4周。于实验前及每周末测量豚鼠双眼屈光度、眼轴数据,实验结束后行脉络膜和巩膜测量。结果 0.01%阿托品组模型眼屈光度迅速下降,实验前后有显著差异[(2.82±1.35)D对(-0.64±0.20)D, P<0.01]。1%阿托品组、优化组1模型眼屈光度下降,实验前后均有统计学差异[(3.50±1.14)D对(1.38±1.15)D, P<0.05;(3.55±1.85)D对(0.95±1.90)D, P<0.01]。优化组2模型眼屈光度下降,实验前后无统计学差异[(1.36±1.61)D对(2.93±1.42)D, P>0.05)]。形觉剥夺后,1%阿托品组眼轴长度无显著变化(P>0.05),其余各干预组眼轴均有不同程度延长。1%阿托品组、优化组2、优化组1脉络膜和巩膜的厚度大于0.01%阿托品组。结论两种优化给药方案的抑制形觉剥夺豚鼠近视作用优于0.01%阿托品,与1%阿托品效果相近。

Objective To study the effect of optimized atropine administration regimen on myopia in guinea pigs.Methods Forty six 21-day old guinea pigs were used for this study. Six were randomly selected as blank control, and the remaining 40 were randomly divided into 5 intervention groups: 1% atropine group, 0.01% atropine group, optimized group 1,optimized group 2, and saline group. One eye of the guinea pig in the intervention groups was randomly selected as the model eye and given form deprivation, and the contralateral eye was the self-control. The duration of intervention was 4 weeks. The diopter and axial length of guinea pig eyes were measured before the experiment and at each weekend. Choroid and sclera were measured after the experiment. Results The diopter of the model eyes in the 0.01% atropine group decreased rapidly. There was a significant difference before and after the experiment [(2.82±1.35)D vs(-0.64±0.20)D, P<0.01]. The diopter of model eyes decreased in 1%atropine group and optimized group 1, and the difference was statistically significant [(3. 50±1.14)D vs(1.38±1.15)D, P<0.05;(3.55±1.85)D vs(0.95±1.90)D, P<0.01]. In optimized group 2, the diopter of model eyes decreased, and there was no significant difference before and after the experiment [(1.36±1.61)D vs(2.93±1.42)D, P>0.05). After form deprivation, the axial length in 1%atropine group did not change significantly(P>0.05). The axial length in other intervention groups was extended to varying degrees.The thickness of choroid and sclera in 1% atropine group, optimized group 1 and optimized group 2 were greater than that in 0.01%atropine group. Conclusion The two optimized dosing regimens worked better than 0.01% atropine in inhibiting myopia in guinea pigs with form deprivation, and were similar to 1% atropine.