氨基酸分析法测定抗HPV功能性妇科敷料中β-乳球蛋白的含量

Determination ofβ-lactoglobulin in Anti-HPV Biological Protein Dressing by Amino Acid Assay

目的建立一种测定抗HPV功能性妇科敷料中β-乳球蛋白含量的氨基酸分析法。方法在酸性条件下将β-乳球蛋白水解为游离氨基酸,采用阳离子交换色谱分离,茚三酮柱后衍生,采集570 nm波长下色谱图,通过测定水解获得的赖氨酸含量,间接测定样品中β-乳球蛋白的含量。结果β-乳球蛋白在77.28~309.12μg/mL浓度范围内有良好的线性关系(y=5.060x+4.278,r=0.9997);加样平均回收率(n=9)为100.06%。结论该方法操作简单、专属性强、准确率高、重复性好,适合于测定抗HPV功能性妇科敷料中β-乳球蛋白的定量分析。

Objective To establish an amino acid assay for the determination ofβ-lactoglobulin in Anti-HPV biological protein dressing.Methods Under acidic conditions,β-lactoglobulin is hydrolyzed into free amino acids,separated by cation exchange chromatography,and derivatived after ninhydrin column.The chromatogram at 570 nm is collected.The content ofβ-lactoglobulin in the sample is indirectly determined by measuring the lysine content obtained by hydrolysis.Resultsβ-lactoglobulin has a good linear relationship in the concentration range of 77.28~309.12μg/mL(y=5.060x+4.278,r=0.9997);The recovery rate(n=9)is 100.06%.Conclusion The method is simple,specific,accurate and reproducible,which is suitable for the quantitative analysis ofβ-lactoglobulin in anti-HPV biological protein dressing.