异黏蛋白与叉头框蛋白M1在肝细胞癌中的表达及其临床意义

Expression and clinical significance of MTDH and FoxM1 in hepatocellular carcinomas

目的:探讨异黏蛋白(MTDH)和叉头框蛋白M1(FoxM1)在肝细胞癌中的表达及其临床意义。方法:收集156例肝细胞癌(HCC)手术切除标本,免疫组织化学和反转录PCR(RT-PCR)分别检测MTDH、FoxM1蛋白和mRNA在HCC中的表达情况,并分析其表达与临床病理指标及预后的关系。结果:免疫组织化学结果显示,MTDH在HCC中的阳性表达率为71.2%(111/156),FoxM1在HCC中的阳性表达率为64.7%(101/156),其表达与肿瘤数目、TNM分期、微血管侵犯、卫星结节等相关(P<0.05)。RT-PCR方法检测显示,MTDH和FoxM1在肝细胞癌中的表达较对照组明显升高(P<0.05)。Spearman相关性分析结果显示,MTDH与FoxM1的表达存在明显的相关性(r=0.507,P<0.01)。Kaplan-Meier生存分析结果显示肝细胞癌患者生存时间与TNM分期、微血管侵犯、卫星结节、MTDH和FoxM1有关,差异均有统计学意义(P<0.05);多因素Cox回归分析结果显示TNM分期和MTDH的表达是影响患者生存时间的主要因素。结论:对于HCC患者,MTDH和FoxM1与诸多临床因素有关,MTDH为患者预后影响因素,且MTDH和FoxM1的表达存在相关性。

OBJECTIVE:To explore relationships between expression of metadherin(MTDH)and forkhead box M1(FoxM1)in hepatocellular carcinomas(HCC)and the clinicopathological features and prognosis of patients.METHODS:Surgical specimens from 156 HCC patients were usedof.Expressions of MTDH and FoxM1 were detected by immunohistochemistry and to analyze the relationship between the expression of MTDH and FoxM1 and the clinical pathological characteristics and prognosis.RESULTS:Immunohistochemical results showed that positive rates of MTDH was 71.2%(111/156).Expressions of MTDH were significantly related to tumor numbers,TNM stages,microvascular invasions,satellite nodules(P<0.05).Positive rates of FoxM1 was 64.7%(101/156).Expressions of FoxM1 were significantly related to cirrhosis,tumor numbers,TNM stages,microvascular invasions,satellite nodules(P<0.05).RT-PCR showed that expression of MTDH and FoxM1 was significantly increased.Spearman correlation analyses showed that there was a significant correlation between MTDH and FoxM1.Results from the Kaplan-Meier survival analyses showed that survival times were significantly related to TNM stages,microvascular invasions,satellite nodules,expressions of MTDH and FoxM1(P<0.05).Results from the multivariate Cox regression analyses showed that TNM stages and MTDH expressions were the main factors which influenced survival times of the patients.CONCLUSION:For HCC patients,MTDH and FoxM1 expressions were related to many clinical factors.Particularly,MTDH expression was a prognostic factor and there was a significant correlation between MTDH and FoxM1 expressions.