皮肤性病60例的人乳头瘤病毒感染型别分析

Analysis of human papillomavirus infection types in 60 cases of skin venereal diseases

目的探讨皮肤性病病人不同病变类型中的人乳头瘤病毒(HPV)感染型别的检测和分析结果。方法选取淮南市第一人民医院2018年2月至2019年2月收治的60例皮肤性病病人作为研究对象,对所有病人均利用PCR-反向斑点杂交法进行HPV-DNA分型的检测,并对皮肤性病病人不同病变类型中HPV感染的型别进行统计和比较。结果本研究60例标本中HPV-DNA检测阳性例数为71.67%(43/60);其中HPV单一型的31例,占比72.09%(31/43);HPV2种或以上者共12例,占比27.91%(12/43)。43例HPV检测阳性标本中高危型检出例数最高,为24例,占比55.81%;低危型13例,占比30.23%;混合型6例,占比13.95%。本研究不同病变类型皮肤性病中,以尖锐湿疣的HPV感染阳性率最高,为76.47%(26/30);鲍温样丘疹病次之阳性率为66.67%(10/15);其次为冠状沟珍珠疹和假性湿疣,阳性率分别为50%(4/8)和42.86%(3/7)。在HPV感染型别上,以尖锐湿疣类别最多,包括了6、11、16、18、31、33、35、43和66,而鲍温样丘疹病主要为16,男性冠状沟珍珠疹主要为低危型的6、42、43和81,女性假性湿疣也为低危型的6、42、83。结论皮肤性病病人HPV感染存在不同的类别,因此临床上在诊断时需要积极开展相应的检测工作,从而为临床治疗方案的设定和预后结果的判断提供参考依据。

Objective To investigate the detection and analysis of human papillomavirus(HPV)infection types in patients with dermatovenereal diseases.Methods Sixty patients with dermatosexually transmitted diseases(STDs)admitted to Huainan First People’s hospital from February 2018 to February 2019 were selected as the research objects,all patients were detected for HPV-DNA typing by PCR-reverse dot hybridization,the types of HPV infection in different pathological types of skin venereal diseases were analyzed and compared.Results The positive rate of HPV-DNA was 71.67%(43/60)in 60 samples,among them,31 cases were single HPV,accounting for 72.09%(31/43);12 cases were HPV or more,accounting for 27.91%(12/43).Among 43 HPV positive specimens,the number of high-risk types was the highest,24 cases,accounting for 55.81%;13 cases of low-risk types,accounting for 30.23%;6 cases of mixed type,accounting for 13.95%.Among the skin and venereal diseases of different pathological types,condyloma acuminatum had the highest positive rate of HPV infection,76.47%(26/30);bowen-like papulosis followed by a positive rate of 66.67%(10/15);followed by coronary pearl rash and pseudocondyloma,with a positive rate were 50%(4/8)and 42.86%(3/7)respectively.Among HPV infection types,condyloma acuminatum was the most types,including 6,11,16,18,31,33,35,43 and 66,while bowen-like papulosis was 16,pearl eruption in male coronary sulcus was 6,42,43 and 81,and pseudocondyloma in female was 6,42 and 83.Conclusion There are different types of HPV infection in patients with dermatosexually transmitted diseases,so it is necessary to actively carry out the corresponding detection work in clinical diagnosis,so as to provide reference for the setting of clinical treatment programs and the judgment of prognosis results.