响应面分析法优化艾叶药渣中白坚木皮醇乙醇提取工艺

Optimization of quebrachitol ethanol extraction from folium Artemisiae argyi extraction dregs by response surface methodology(RSM)

以白坚木皮醇的提取率为指标,通过单因素实验和响应面分析法对艾叶药渣中白坚木皮醇的乙醇提取工艺条件进行优化研究,考查了提取时间、料液比、提取温度、提取次数对白坚木皮醇提取率的影响,使用Box-Behnken试验设计方法对影响白坚木皮醇提取率的各因素进行显著性及交互作用分析,最终确定从艾叶药渣中提取白坚木皮醇的最佳工艺条件。结果表明,提取艾叶药渣中白坚木皮醇的最佳工艺条件为:每次提取时间2.5h,提取次数3次,料液比1∶25,提取温度80℃,在这个最佳提取条件下白坚木皮醇提取率可达1.765±0.003%。实际值与预测值之间被证明具有很好的拟合性,因此该提取工艺条件可提高白坚木皮醇的提取率。研究结果指出了一条获取白坚木皮醇的新途径,提高了艾叶的附加值。

Single factor experiments and response surface analysis were used to optimize ethanol extraction process of quebrachitol from folium Artemisiae argyi extration dregs.Quebrachitol extraction rate was the index.The effects of extraction times,solid-liquid ratio,extraction temperature,and extraction times on the extraction rate were studied by Box-Behnken design.The influence factors on the extraction rate rate of quebrachitol were determined.The results showed that the optimal process conditions were:extraction 2.5 h for 3 times,material-to-liquid ratio 1∶25,extraction temperature 80℃.Under the above conditons,the extraction rate can reach to 1.765±0.003%.The study verified experiement results and predicted value have a good fit.The new way of obtaining quebrachitol is practical and it adds more value to folium Artemisiae argyi.