响应面分析法优化超临界萃取烟末中的茄尼醇

Response Surface Optimization of Supercritical CO_(2) Extraction of Solanesol from Tobacco Residue

采用Box-Benhnken实验设计及响应分析法对茄尼醇萃取过程中的萃取压力、萃取温度和夹带剂流量3个因素进行优化.结果显示,夹带剂流量对萃取率的影响最大,萃取压力次之,萃取温度影响最小,最佳的参数为:萃取压力19.1 MPa、萃取温度51.3℃和夹带剂流量0.19 mL/min;在此条件下进行验证,茄尼醇的平均萃取率为69%.萃取物通过柱色谱分离,用石油醚、乙酸乙酯洗脱,浓缩并重结晶后得到纯度为93.2%的高纯度茄尼醇,茄尼醇收率为0.44%.

Box-Benhnken experimental design and response analysis were used to optimize the extraction pressure,extraction temperature and entrainer flow rate in Solanesol extraction process.The results showed that entrainer flow rate had the greatest effect on the extraction rate,followed by extraction pressure,while the extraction temperature had the least impact,the optimal parameters were as follows:extraction pressure 19.1 MPa,extraction temperature 51.3℃,entrainer flow rate 0.19 mL/min.Under this condition,the average extraction rate of Solanesol was 69%.The extract was separated by column chromatography,eluted with petroleum ether and ethyl acetate,concentrated and recrystallized to obtain high-purity Solanesol with purity of 93.2%,and the yield of Solanesol was 0.44%.