九龙藏黄牛和九龙牦牛β-酪蛋白基因型分析

Genotyping on β-caseins of Jiulong Tibetan Yellow Cattle and Jiulong Yak

为比较九龙藏黄牛和九龙牦牛β-酪蛋白(β-CN)的遗传变异体,试验采用酸性尿素聚丙烯酰胺凝胶电泳分析了九龙藏黄牛(n=42)和九龙牦牛(n=17)β-CN的基因型。结果表明,在九龙藏黄牛、九龙牦牛的β-CN中共检测到4种等位基因,包括A1、A2、B、C,其中在九龙藏黄牛中有7种基因型:A1A1、A1A2、BB、A1B、A2B、A1C、BC,优势等位基因为A1(频率0.7024),优势基因型为A1A1(频率0.5476);在九龙牦牛样本中有2种基因型:A1A2、A2A2,优势等位基因为A2(频率0.7647)。试验表明,九龙藏黄牛与九龙牦牛β-CN均表现出多态性,但优势等位基因明显不同。

The β-casein(β-CN)variants were identified in Jiulong Tibetan Yellow cattle and Jiulong yak by acid urea polyacrylamide gel electrophoresis.The results indicated that four alleles,including A1,A2,B and C,were detected in β-CN of Jiulong Tibetan Yellow cattle and Jiulong yak.There were 7 genotypes in Jiulong Tibetan yellow cattle:A1A1,A1A2,BB,A1B,A2B,A1C and BC,the dominant allele was A1(the frequency was 0.7024),and the dominant genotype was A1A1(the frequency was 0.5476).There were two genotypes in Jiulong yak:A1A2 and A2A2,and the dominant allele was A2(the frequency was 0.7647).This study showed that the β-CN polymorphisms were found in both Jiulong Tibetan yellow cattle and Jiulong yak,but the dominant alleles were significantly different.