基于转录组测序的苦荞FtGLT鉴定与表达分析

Gene Identification and Expression Analysis of FtGLT in Tartary Buckwheat Based on Transcriptome Sequencing

植物糖类转运蛋白家族中GLT(Glucose transporter)是介导葡萄糖跨膜运输的重要载体。开展苦荞葡萄糖转运蛋白(FtGLT)的生物信息学研究,可为进一步探索FtGLT调控生长发育与产量形成提供科学参考。通过基因注释与序列比对分析,在苦荞转录组测序数据库中筛选到6个GLT同源基因。FtGLT序列在2167~9707 bp间,编码94~558个氨基酸残基。FtGLT蛋白间序列的一致性达到43.44%,说明苦荞FtGLT蛋白序列相对保守。除FtPinG68303.01外,其余5个FtGLT蛋白可能位于内质网。结构分析发现,苦荞FtGLT蛋白具有数量不等的跨膜结构域,这与其结构中α-螺旋数量密切相关。基于转录组测序,鉴定到3个FtGLT显著差异表达基因,均在出苗后15 d高表达,其余3个基因表达量在5、10和15 d变化较小,推测不同FtGLT基因在茎发育过程中的功能可能存在差异。相关性分析发现,FtPinG68303.01与FtPinG50279.01呈极显著负相关;FtPinG83070.01与FtPinG89831.01和FtPinG72784.01,FtPinG89831.01与FtPinG72784.01呈极显著或显著正相关,这可为进一步研究苦荞FtGLT相互间的功能及其调控葡萄糖运输的分子机制提供依据。

GLT(Glucose transporter)is an important carrier in plant glucose transporter family.Bioinformatics analysis of FtGLT in Tartary buckwheat(Fagopyrum tataricum)can provide scientific reference for further exploration of plant growth and development and yield formation regulation by FtGLT.A total of 6 GLT homologous genes of Tartary buckwheat were screened by gene annotation and sequence alignment analysis.The FtGLT genes sequence length were ranged from 2167 to 9707 bp,and the encoded amino acid residues were ranged from 94 to 558 aa.The sequence consistency of FtGLT proteins was 43.44%,indicating that the FtGLT protein sequence of Tartary buckwheat was relatively conservative.Except for FtPing68303.01,the other 5 FtGLT proteins were predicted to be located in the endoplasmic reticulum.Protein structure analysis indicated that FtGLT contained different transmembrane domains,of which number was closely related to theα-helix numbers.A total of 3 differentially expressed genes were screened,of which expression levels were highly detected at 15 d after seedling emergence,based on transcriptome sequencing.By contrast,the expression levels of the other three genes changed slightly at 5,10 and 15 d,suggesting that the different functions of FtGLT genes might occur in stem during development.Correlation analysis showed an extremely significantly negative correlation between FtPing68303.01 and FtPing50279.01,whereas the extremely significantly or significantly positive correlation among FtPing830-70.01,FtPing89831.01 and FtPing72784.01,between FtPing89831.01 and FtPing72784.01.The results of this study provide a basis for further study of the functions of Tartary buckwheat FtGLT and the molecular mechanisms of glucose transport regulation.