蔗茅EfNAC基因的克隆和生物信息学分析

Cloning and Bioinformatics Analysis of The EfNAC Gene in Erianthus fulvus

为了更好地利用甘蔗野生种蔗茅(Erianthus fulvus Ness.)的抗逆相关基因,并为转基因甘蔗抗逆育种提供可靠的候选基因。本研究利用电子克隆和RT-PCR的方法在蔗茅野生种‘蔗茅99-2’中克隆到一个干旱诱导表达基因,并命名为FfNAC(GenBank登录号:MT499790)。生物信息学分析表明,FfNAC基因ORF长度为765 bp,共编码254个氨基酸,具有一个保守的NAM结构域。编码的蛋白以无规则卷曲和α-螺旋为主,没有信号肽,具有多个磷酸化位点,没有跨膜结构。本研究为深入研究蔗茅干旱胁迫的分子调控机制和转基因甘蔗抗旱育种提供理论依据和技术支撑。

In order to make better use of the resistance-related genes of sugarcane wild species Erianthus fulvus Ness.,and to provide reliable candidate genes for resistance breeding of transgenic sugarcane.In this research,we used electronic cloning and RT-PCR to clone a gene that was induced by drought stress in the wild species of E rianthus fulvus'Erianthus fulvus 99-2',and named it FfNAC(GenBank ID:MT499790).Bioinformatics analysis showed that the ORF of the FfNAC is 765 bp in length,encodes 254 amino acids in total,and has a conserved NAM Superfamily.FfNAC protein is mainly composed of random coils and alpha helices,without signal peptide,with multiple phosphorylation sites,and no transmembrane structure.This research provides theoretical basis and technical support for in-depth research of the molecular regulation mechanism of drought stress in the sugarcane and the drought-resistant breeding of transgenic sugarcane.