转化生长因子β1基因509C/T、869T/C多态性与原发性肝癌的关系

Association of the TGF-β1-509C/T,869T/C polymorphisms with primary hepatocellular carcinoma

目的:探讨转化生长因子β1(TGF-β1)基因509C/T、869T/C单核苷酸多态性与原发性肝癌的关系。方法:选取我院初诊收治的原发性肝癌(primary liver cancer,PLC)患者112例为研究对象(PLC组),另取同期年龄、性别匹配健康体检者115例为对照(对照组),采用TaqMan-MGB探针等位基因分型技术对TGF-β1-509C/T、869T/C单核苷酸多态性进行检测,分析其多态性与PLC易感性、环境交互作用及生存预后的关系,并进行统计学分析。结果:509C/T位点多态性与PLC易感性无显著关联(P>0.05),869T/C位点携带CC型基因会显著增加PLC风险(OR=2.106,95%CI:1.513~3.329,P=0.031),合并分析C等位基因是T等位基因PLC发病风险2.539倍(95%CI:1.528~3.357,P=0.026)。509C/T位点CT/TT型基因与HBV感染有显著交互作用(OR:2.677,95%CI:1.921~3.688,P=0.012),869T/C位点CC/TC型基因与饮酒(OR:4.128,95%CI:2.173~5.896,P=0.006)、HBV感染(OR:5.792,95%CI:3.623~6.817,P=0.000)有显著交互作用。509C/T位点各基因型PLC患者中位生存时间无显著差异(P>0.05),869T/C位点CC/TC型基因中位生存时间显著低于TT型(21个月、27个月,P=0.007)。结论:TGF-β1-509C/T多态性与PLC易感性无关,与HBV存在交互作用;869T/C位点C等位基因可能是PLC易感基因,与饮酒、HBV感染存在交互作用,对PLC预后有一定影响。

Objective:To explore the association of the TGF-β1-509C/T,869T/C polymorphisms with primary hepatocellular carcinoma.Methods:112 cases of primary liver cancer(PLC)who have been diagnosed in our hospitals were selected as the subjects(PLC group),and 115 cases of matched healthy persons were taken as the control group(control group).The TaqMan-MGB probe allele typing was used to detected the single nucleotide polymorphisms of TGF-β1-509C/T,869T/C.The relationship between polymorphisms and PLC susceptibility,environmental interaction and survival prognosis was statistically analyzed.Results:There were no significant association between the polymorphism of 509C/T and PLC susceptibility.The CC allele of 869T/C could significant increase the risk on PLC(OR=2.106,95%CI:1.513~3.329,P=0.031),and the combined analysis of C allele was 2.539 times the risk of T allele(95%CI:1.528~3.357,P=0.026).The CT/TT allele of 509C/T had significant interaction with HBV infection(OR:2.677,95%CI:1.921~3.688,P=0.012),and the CC/TC allele of 869T/C had significant interation with drinking(OR:4.128,95%CI:2.173~5.896,P=0.006)and HBV infection(OR:5.792,95%CI:3.623~6.817,P=0.000).There was no significant difference in the median survival time of the PLC patients with 509C/T,and the median survival time of CC/TC allele was significantly lower than that TT allel in 869T/C gene(respectively,21 months,27 months,P=0.007).Conclusion:TGF-β1-509C/T polymorphism is not related to PLC susceptibility,and has interaction with HBV.The C allele of 869T/C may be a susceptible gene of PLC,interacting with drinking and HBV infection,and has a certain effect on the prognosis of PLC.