瞬时受体蛋白通道C5和mi R-320a在甲状腺癌中的表达及临床意义

Expressions of TRPC5 and miR-320a in thyroid cancer and their clinical significances

目的检测瞬时受体蛋白通道C5(TRPC5)和微小RNA-320a(miR-320a)在甲状腺癌组织中的表达并探讨二者在甲状腺癌中表达的临床意义。方法通过检索Ualcan数据库以了解甲状腺癌中TRPC5和miR-320a mRNA的表达情况;检测郑州市第七人民医院2014年3月至2015年3月期间的80例甲状腺癌组织、35例甲状腺腺瘤组织及32例甲状腺正常组织中TRPC5和miR-320a mRNA的表达情况,采用实时荧光定量PCR技术检测各组织样本中TRPC5、miR-320a mRNA的表达情况并分析二者与甲状腺癌患者临床病理特征的关系;采用Western blot法检测TRPC5蛋白的表达情况;TRPC5和miR-320a mRNA表达的相关性分析采用Pearson简单相关分析;采用Kaplan-Meier法绘制生存曲线,同时采用单因素和多因素Cox比例风险回归模型分析影响患者预后的危险因素。结果在Ualcan数据库中发现,甲状腺癌组织中TRPC5 mRNA的表达水平高于正常甲状腺组织(P<0.001),而miR-320a mRNA的表达水平则低于正常甲状腺组织(P<0.001)。与正常甲状腺组织相比,甲状腺癌组织中TRPC5 mRNA的表达水平增高(P<0.05),而miR-320a mRNA的表达水平则降低(P<0.05),且二者在甲状腺癌组织中的表达呈负相关(r=–0.653,P<0.001);TRPC5、miR-320a mRNA表达与甲状腺癌患者的肿瘤分化程度、淋巴结转移和TNM分期有关(P<0.05)。TRPC5 mRNA高表达和miR-320a mRNA低表达患者的预后不良,且多因素分析结果发现肿瘤分化程度、TNM分期、淋巴结转移、TRPC5 mRNA高表达和miR-320a mRNA低表达是影响甲状腺癌患者预后的危险因素(P<0.05)。结论从数据库和临床病例数据均得出,在甲状腺癌组织中TRPC5 mRNA高表达、miR-320a mRNA低表达,且二者表达与甲状腺癌患者的肿瘤分化程度、淋巴结转移、TNM分期等临床病理特征及预后有关,有可能是甲状腺癌临床及预后监测的潜在指标。

Objective To detect expressions of transient receptor potential channel C5(TRPC5)and microRNA-320 a(miR-320 a)in thyroid cancer and explore clinical significances of them in thyroid cancer.Methods The expressions of TRPC5 and miR-320 a mRNA in the thyroid cancer were investigated by searching the Ualcan database.While the expressions of TRPC5 and miR-320 a mRNA in 80 cases of thyroid cancer,35 cases of thyroid adenoma and32 cases of normal thyroid tissues adjacent to thyroid adenoma tissues in the Zhengzhou Seventh People’s Hospital from March 2014 to March 2015 were tested.Real time PCR was used to detect the expressions of TRPC5 mRNA and miR-320 a mRNA in the various tissues and Western blot was used to detect the TRPC5 protein in the thyroid cancer tissues.The relationships between the expressions of TRPC5 and miR-320 a mRNAs and clinicopathologic features of thyroid cancer were analyzed.The correlation between expressions of TRPC5 and miR-320 a mRNA was analyzed by Pearson method.The risk factors influencing the prognosis were analyzed by univariate and multivariate Cox proportional hazards regression model.Results The results of Ualcan database showed that the expression level of TRPC5 mRNA in the thyroid cancer was higher than that in the normal thyroid tissue(P<0.001),while the expression level of miR-320 a mRNA was lower than that in the normal thyroid tissue(P<0.001).The results of clinical cases showed that the expression level of TRPC5 mRNA was significantly higher,while the expression of miR-320 a mRNA was significantly lower in the thyroid cancer tissues as compared with the normal thyroid tissues(P<0.05).There was a negative correlation between the expression level of TRPC5 and miR-320 a mRNA in the thyroid cancer(r=-0.653,P<0.001).The expressions of TRPC5 and miR-320 a mRNA were correlated with the degree of differentiation,lymph node metastasis,and TNM stage(P<0.05).Kaplan-Meier survival curve analysis found that the patients with higher expression level of TRPC5 and lower expression level of miR-320 a showed the poor prognosis,and multivariate analysis found that the lower tumor differentiation,later TNM stage,with lymph node metastasis,higher expression level of TRPC5 mRNA,and lower expression level of miR-320 a mRNA were the risk factors affecting prognostic survival(P<0.05).Conclusions From the database and clinical case data,it is concluded that TRPC5 mRNA is highly expressed,while miR-320 a mRNA is lowly expressed in thyroid cancer tissues,and expressions of TRPC5 and miR-320 a mRNA are related to degree of tumor differentiation,lymph node metastasis,TNM staging,and prognosis in patients with thyroid cancer.TRPC5 and miR-320 a mRNA might be used as potential indicators for clinical and prognostic monitoring.