魔芋粉通过AMPK信号通路调控肥胖大鼠脂质代谢作用

Regulative effect of konjac flour on lipid metabolism through AMPK signaling pathway and its mechanism in obese rats

目的探讨魔芋复方对腺苷酸蛋白活化激酶(AMPK)相关信号转导通路的影响,了解其调节脂质代谢(减肥作用)机制。方法以SD大鼠为研究对象,建立肥胖模型,将实验动物分为对照组、模型组和魔芋组,采用试剂盒法检测大鼠血清甘油三酯(TG)、胆固醇(TC)、AMPK、脂肪酸合成酶(FAS)、HMG-CoA还原酶(HMGCR)、乙酰辅酶A羧化酶(ACC)的水平。结果与对照组比较,模型组大鼠血清TG、TC含量升高,AMPK活性下降,FAS、HMGCR活性升高;与模型组比较,高剂量魔芋组大鼠血清TG含量(1.08±0.13)mmol/L、TC含量(2.02±0.31)mmol/L明显降低(P<0.05),高剂量魔芋组大鼠血清MAPK表达水平(2014.45±724.25)升高(P<0.05),FAS、HMGCR表达水平[分别为(13.48±6.98)、(40.47±4.35)]降低(P<0.05);高、中剂量魔芋组大鼠血清ACC表达水平[分别为(125.75±6.75)、(126.02±7.15)]降低(P<0.05)。结论魔芋复方能够依靠AMPK调脂通路影响脂质代谢,起到干预肥胖的作用。

Objective To explore the effect of konjac compound(konjac) on AMP-activated protein kinase(AMPK)-related signal transduction pathways and the mechanism of the compound in regulating lipid metabolism(weight loss effect)in rats. Methods Obesity model was established in 60 Sprague-Dawley(SD) rats and the rats were randomly assigned into 6 groups(10 in each group): a control group, a model group, three konjac groups at dosages of 0.344, 0.172, 0.086 g/kg, and a positive control group with orlistat capsules. By the end of the 6-week treatment, the rats′ serum triglyceride(TG), total cholesterol(TC), AMPK, fatty acid synthesis(FAS), 3-hydroxy-3-methylglutaryl coenzyme-A reductase(HMGCR), and acetyl-CoA carboxylase(ACC) were detected with kit method. Results In comparison with those in the control rats, the serum TG,TC, and FAS and HMGCR activity increased but AMPK activity decreased in the model rats. Commpared with the model rats, the high dose konjac-treated rats had significantly reduced serum content of TG(1.08 ± 0.13) and TC(2.02 ±0.31), expression of FAS(13.48 ± 6.98) and HMGCR(40.47 ± 4.35), but elevated serum AMPK expression(2014.45 ±724.25)(P < 0.05 for all);in addition, the high and moderate dose konjac-treated rats had significantly decreased the serum ACC expression(125.75 ± 6.75 and 126.02 ± 7.15)(both P < 0.05). Conclusion Konjac compound could regulate lipid metabolism through AMPK signaling pathway and impact on obesity in rats.