芽胞杆菌中重组蛋白分泌表达的优化策略及研究进展

Optimization strategy and research progress of recombinant protein secretory expression in Bacillus

芽胞杆菌属具有良好的蛋白表达和分泌能力,在工业酶的生产中被广泛应用,是理想的工业宿主菌,但实现蛋白分泌表达的普遍高效性还存在许多瓶颈。本文综述了芽胞杆菌的蛋白分泌表达策略,从启动子、信号肽、分泌途径、宿主和培养条件这5个方面总结了提高芽胞杆菌中分泌表达重组蛋白的方法,对芽胞杆菌高效生产工业酶有一定的参考价值,最后展望了优化芽胞杆菌分泌表达的研究方向,各种新型生物技术的发展必将推进芽胞杆菌在分泌表达领域有更深入的应用。

Bacillus has good protein expression and secretion ability,and is widely used in the production of industrial enzymes.There are still many bottlenecks to achieve protein secretory expression with a generally high efficiency,although it is an ideal industrial host strain.In this review,the secretion pathway of protein in Bacillus is briefly described,and we summarize the strategies of host strain optimization for recombinant protein production from five different aspects,including promoter,signal peptide,secretion pathway,host strain and culture conditions,which has certain reference value for the efficient production of industrial enzymes in Bacillus.The research direction of optimizing secretory expression of Bacillus in the future is prospected.The development of various new biotechnology will certainly promote the further application of Bacillus in secretory expression.