基于乙酰胆碱酯酶传感器检测玉米中黄曲霉毒素B_(1)

Detection of aflatoxin B_(1) in corn based on acetylcholinesterase sensor

本实验制备了氮掺杂碳球复合纳米铂纳米复合材料(N-Cs@Pt),并在此基础上构建了乙酰胆碱酯酶(AChE)传感器N-Cs@Pt/AChE/GCE,对玉米中的黄曲霉毒素B1(AFB1)进行定量分析。结果表明:N-Cs@Pt能显著改善N-Cs@Pt/AChE/GCE的导电性,提高传感器的灵敏度。利用N-Cs@Pt/AChE/GCE对AFB1进行定量分析,AFB1浓度与其对传感器的抑制率在1~30μg/mL内呈良好的线性关系,线性方程为Y=0.9618X+30.214,R^(2)=0.9989,检出限为0.23μg/mL(S/N=3),其加标回收率为97.83%~102.88%,与HPLC所得到的结果一致,准确度较高。且该传感器稳定性较好,42 d后其催化效率仍可达到正常传感器的95.5%。该方法简单、便捷,可用于玉米中AFB1的快速定量分析。

Nitrogen doped carbon spheres composite platinum nanocomposites were prepared(N-Cs@Pt),and the acetylcholinesterase(AChE)sensor N-Cs@Pt/AChE/GCE was constructed based on N-Cs@Pt for detecting aflatoxin B1(AFB1)in corn.The study found that N-Cs@Pt could significantly improve N-Cs@Pt/AChE/GCE conductivity and increase sensor sensitivity.Quantitative analysis of AFB1 was done,the results showed that there was a good linear relationship between the concentration of AFB1 and the inhibition rate of the sensor in the range of 1~30μg/mL,the linear formula was Y=0.9618X+30.214,R^(2)=0.9989,the detection limit was 0.23μg/mL(S/N=3),and the recoveries were 97.83%~102.88%,which was consistent with the results obtained by HPLC.And the stability of the sensor was good,and its catalytic efficiency could still reach 95.5%of the normal sensor after 42 days.The method was simple and convenient,and could be used for rapid quantitative analysis of AFB1 in maize.