老年晚期宫颈癌患者贝伐珠单抗治疗后外周血miR-21、miR-130表达水平变化及临床意义

The Expression Level and Clinical Significance of Peripheral Blood miR-21 and miR-130 in Elderly Patients with Advanced Cervical Cancer After Bevacizumab Treatment

目的探讨老年晚期宫颈癌患者贝伐珠单抗治疗后外周血miR-21、miR-130表达水平变化及临床意义。方法选取化疗耐药的老年晚期宫颈癌患者63例为研究对象,使用贝伐珠单抗注射液进行治疗。采用RT-PCR技术检测患者治疗前后外周血中miR-21、miR-130表达水平,分析miR-21、miR-130水平与贝伐珠单抗治疗效果的关系;采用Kaplan-Meier生存曲线分析外周血miR-21、miR-130表达水平与生存率的关系。结果老年晚期宫颈癌患者经贝伐珠单抗治疗后患者外周血miR-21、miR-130表达水平明显下降(P<0.001);经贝伐珠单抗治疗后不同治疗效果患者外周血中miR-21、miR-130水平具有明显差异(P<0.001),且CR组、PR组外周血中miR-21、miR-130水平明显低于PD组(P<0.05)。外周血miR-21、miR-130高表达患者生存率低于低表达患者(P<0.05)。结论老年晚期宫颈癌患者外周血miR-21、miR-130水平在经贝伐珠单抗治疗后明显下降,其可能作为贝伐珠单抗疗效及患者预后的相关指标发挥临床作用。

Objective To investigate the expression levels of miR-21 and miR-130 in peripheral blood of elderly patients with advanced cervical cancer after bevacizumab treatment and their clinical significance.Methods 63 patients with chemotherapy-resistant advanced cervical cancer were selected as the research objects,and bevacizumab injection was used for treatment.RT-PCR technology was used to detect the expression levels of miR-21 and miR-130 in the peripheral blood of patients before and after treatment,and the relationship between miR-21 and miR-130 levels and the treatment effect of bevacizumab was analyzed.The Kaplan-Meier survival curve was used to analyze the peripheral blood.The relationship between blood miR-21,miR-130 expression level and survival rate.Results The expression levels of miR-21 and miR-130 in peripheral blood of elderly patients with advanced cervical cancer were significantly decreased after bevacizumab treatment(P<0.001);miR-in peripheral blood of patients with different treatment effects after bevacizumab treatment 21.The levels of miR-130 were significantly different(P<0.001),the levels of miR-21 and miR-130 in the peripheral blood of the CR and PR groups were significantly lower than those of the PD group(P<0.05);The 36-month survival rate of miR-130 high expression patients was lower than that of low expression patients(P<0.05).Conclusion The levels of miR-21 and miR-130 in peripheral blood of elderly patients with advanced cervical cancer decreased significantly after treatment with bevacizumab,which may play a clinical role as a relevant indicator of the efficacy of bevacizumab and the prognosis of patients.