轮状病毒G9P[8]-E2基因型重配株在我国的流行趋势分析

Spread trend analysis on reassortant of rotavirus G9P[8]-E2 in China

目的研究我国轮状病毒(RV)流行毒株G9P[8]基因型的全序列分子特征及进化规律。方法采用反转录–聚合酶链式反应(RT-PCR)方法,对2018年收集的64份(广东省深圳市和吉林省长春市各32份)G9P[8]基因型RV阳性标本进行扩增。结果成功获取54份(深圳市30份,长春市24份)G9P[8]基因型A组轮状病毒(RVA)标本的全基因组节段。序列分析表明,54份G9P[8]基因型RVA中, 28份为Wa-like G9P[8]-E1株,26份为非结构蛋白NSP4基因节段重配的G9P[8]-E2株,G9P[8]-E2株在深圳和长春市阳性样本中分别占60.0%、33.3%。结论 2018年深圳和长春市流行的RVA中G9P[8]-E2重配株已经成为优势株,且在深圳市的优势程度大于在长春市的,推测该基因型重配株RVA可能已在我国广泛传播。

Objective To study the full sequence molecular and evolutionary characteristics of rotavirus G9P[8] in China.Methods A total of 64 rotavirus G9P[8] positive samples collected in 2018(32 from Shenzhen of Guangdong province and Changchun of Jilin province, respectively) were amplified by reverse transcriptase polymerase chain reaction(RT-PCR).Results The whole genome segments of 54 of rotavirus group A(RVA) G9P[8] isolates(30 isolates from Shenzhen and 24 isolates from Changchun) were amplified successfully. Sequence analysis showed that among the 54 RVA G9P[8] strains,there were 28 Wa-like strains of G9P[8]-E1 type(G9-P[8]-I1-R1-C1-M1-A1-N1-T1-E1-H1), 26 NSP4-segment reassortant G9P[8]-E2 strains(G9-P[8]-I1-R1-C1-M1-A1-N1-T1-E2-H1). The reassortant strains in Shenzhen and Changchun accounted for 60.0% and 33.3%, respectively. Conclusion RVA G9P[8]-E2 reassortant has become the predominant strain in RVA detected in the south and north of China in 2018, which was more obvious in southern China than in northern China. It is speculated that reassortant of RVA G9P[8]-E2 might have widely spread in China.