丝状真菌代谢组学研究方法进展

Advances in research methods of filamentous fungal metabolomics

代谢组学研究的是生物体系受到内在和外在因素刺激产生的内源性代谢变化。近年来,随着代谢组学在病原真菌致病机理、真菌与植物互作以及新型生物活性物质开发研究领域的应用,真菌代谢组学越来越受到国内外的重视,同时也取得了较大的进展。由于丝状真菌类型和研究目的的差异,其研究方法不尽相同。本文主要综述近年来国内外丝状真菌代谢组学分析方法的研究进展,从丝状真菌样品制备、代谢物鉴定、数据分析、生物标志物发掘、代谢通路方面进行概述,以期为该领域研究者提供参考,进一步推进代谢组学在真菌研究领域中的应用。

Metabolomics studies the endogenous metabolic changes in biological systems stimulated by internal and external factors.In recent years,with the application of metabolomics in the pathogenesis of pathogenic fungi and interaction with plants,the development of novel biologically active substances,more and more attentions have been paid to the study of fungal metabolomics,and great progresses have been made in this area.Due to the differences in the types of filamentous fungi and the purposes of the research,the research methods were diverse.This review summarized the research progresses in the metabolomic analysis methods of filamentous fungi in recent years,including filamentous fungal sample preparation,metabolite identification,data analysis,biomarker discovery and metabolic pathways.It is aimed to provide a reference for the researchers in this field and further promote the application of metabolomics to fungal research.