妊娠期糖尿病患者口腔菌群和肠道菌群特征分析

The analysis of oral and intestinal microbiota features in patients with gestational diabetes

目的:探讨口腔菌群和肠道菌群与妊娠期糖尿病(GDM)的关系。方法:利用16S rRNA扩增子测序技术分析中孕期GDM患者(n=11)和健康对照(n=40)口腔菌群和肠道菌群的特征。结果:与健康组相比,GDM组唾液中气单胞菌属和代尔夫特菌属,牙菌斑中气单胞菌属,肠道中嗜血杆菌属的丰度显著降低(P<0.05)。牙龈卟啉单胞菌和多雷类杆菌的丰度分别与OGTT-0 h以及OGTT-1 h血糖水平显著正相关(r=0.243,P=0.001;r=0.206,P=0.005)。结论:牙龈卟啉单胞菌和多雷类杆菌可能影响中孕期妇女血糖水平。

Objective:To investigate the relationship between oral and intestinal microflora and gestational diabetes mellitus(GDM).Methods:Features of oral and intestinal microflora from 11GDM patients and 40 healthy controls were analyzed with 16S rRNA amplicon sequencing technique.Results:Compared to their control group,the abundance of Aeromonas in saliva and dental plaque,Delftia and Porphyromonas in saliva and Haemophilus in intestine were significantly decreased in GDM groups(P<0.05).Significant correlations were found between Porphyromonas gingivalis abundance and glucose levels of OGTT-0 h(r=0.243,P=0.001),and between Bacteroides dorei and glucose levels of OGTT-1 h(r=0.206,P=0.005).Conclusion:Porphyromonas gingivalis and Bacteroides dorei might be the influence factors to glucose levels of women in middle pregnancy.