摘要
CRISPR-mediated genome editing has been widely applied in plants to make uncomplicated genomic modifications including gene knockout and base changes.However,the introduction of many genetic variants related to valuable agronomic traits requires complex and precise DNA changes.Different CRISPR systems have been developed to achieve efficient sequence insertion and replacement but with limited success.A recent study has significantly improved NHEJ-and HDR-mediated sequence insertion and replacement using chemically modified donor templates.Together with other newly developed precise editing systems,such as prime editing and CRISPR-associated transposases,these technologies will provide new avenues to further the plant genome editing field.
基金
This work was supported by the National Science Foundation Plant Genome Research Program grants(award nos.IOS-1758745 and IOS-2029889)
Biotechnology Risk Assessment Grant Program competitive grant(award no.2018-33522-28789)
from the US Department of Agriculture,Syngenta,and Foundation for Food and Agriculture Research grant(award no.593603).