低分子肝素对子宫内膜容受性的作用及其机制研究

Effect of low molecular weight heparin on endometrial receptivity and its mechanism

目的研究低分子肝素(LMWH)影响子宫内膜容受性的作用机制。方法随机将C57BL/6小鼠分为对照组、造模组、LMWH低剂量造模组和LMWH高剂量造模组。妊娠第5天统计胚胎数量,HE染色对比各组子宫内膜腺体及血管情况,qRT-PCR检测血小板内皮细胞黏附因子1(PECAM1)、同源框基因A10(HOXA10)、WNT7A和β-catenin的mRNA水平。结果LMWH低剂量造模组和LMWH高剂量造模组胚胎数量较造模组增加(P<0.05),LMWH高剂量造模组最高。LMWH高剂量造模组胚胎数量较对照组增加(P<0.05)。LMWH低剂量造模组和LMWH高剂量造模组腺体、血管数量较造模组增加(P<0.05),而LMWH造模组与对照组比较,差异无统计学意义(P>0.05)。LMWH低剂量造模组和LMWH高剂量造模组HOXA10、PECAM1、Wnt7A、β-catenin mRNA相对表达量较造模组增加(P<0.05)。结论LMWH通过Wnt通路提高子宫内膜容受性,其作用机制可能与PECAM1相关。

Objective To study the effect and mechanism of low molecular weight heparin(LMWH)on endometrial receptivity.Methods The blastocyst implantation failure model was established via mifepristone,and C57 BL/6 mice were randomly divided into control group,model group,low-dose LMWH model group and highdose LMWH model group.The number of embryos on the fifth day of gestation was counted.HE staining was used to compare endometrial glands and blood vessels in each group,and qRT-PCR was applied to detect mRNA levels of platelet endothelial cell adhesion molecule 1(PECAM1),homeobox A10(HOXA10),Wnt family member 7A(WNT7A)and beta-catenin.Results Compared with the model group,the number of embryos was increased in the low-dose LMWH model group and high-dose LMWH model group and was even greater in the high-dose LMWH model group(P<0.05).Besides,the number of embryos in the high-dose LMWH model group was also greater than that in the control group(P<0.05).The number of endometrial glands and blood vessels in the low-dose LMWH model group and high-dose LMWH model group was greater than that in the model group(P<0.05),but was not different compared with that in the control group(P>0.05).In addition,mRNA expression levels of PECAM1,HOXA10,WNT7A and beta-catenin in the low-dose LMWH model group and high-dose LMWH model group were higher compared with the model group(P<0.05).Conclusions LMWH improves endometrial receptivity through WNT pathway,and its mechanism may be related to PECAM1.