MCP-1和CD105在稽留流产患者蜕膜组织中的表达及意义

Expression of MCP-1 and CD105 in the decidual tissue of patients with missed abortion and their significance

目的探讨单核细胞趋化蛋白-1(MCP-1)和CD105在稽留流产(MA)患者蜕膜组织中的表达及意义。方法选取2020年6至12月重庆医科大学附属永川医院收治住院的10例MA患者作为MA组,10例正常宫内早孕要求终止妊娠者作为对照组,提取两组蜕膜免疫细胞(DIC),利用流式细胞术检测其MCP-1阳性细胞比例,免疫组化法观察蜕膜组织中CD105的定位及表达水平,Western blot法检测蜕膜组织中CD105的表达水平。结果与对照组比较,MA组患者DIC中MCP-1阳性细胞比例明显升高(P<0.05)。免疫组化结果显示,两组蜕膜基质细胞的细胞质与细胞核中均有CD105表达,MA组CD105表达水平较对照组明显升高(P<0.05)。Western blot结果显示,与对照组比较,MA组蜕膜组织中CD105蛋白表达水平升高(P<0.05)。两组MCP-1阳性细胞比例与CD105蛋白表达水平均呈正相关(r=0.931和0.940,均P<0.01)。结论MCP-1和CD105在MA患者蜕膜组织中表达水平升高,促进了MA的发生和发展。

Objective To explore the expression and significance of monocyte chemoattractant protein-1(MCP-1)and CD105 in the decidual tissue of patients with missed abortion(MA).Methods From June to December 2020,10 patients with MA admitted to the Yongchuan Hospital of Chongqing Medical University were enrolled(MA group),and 10 women with normal intrauterine pregnancy who required termination of pregnancy were selected as the control group.Dedidual immunocyte(DIC)of two groups was extracted,and the proportion of MCP-1 positive cells was detected by flow cytometry,the localization and expression level of CD105 in decidual tissue were detected by immunohistochemistry,the expression level of CD105 in decidual tissue was detected by Western blot.Results Compared with the control group,the proportion of MCP-1 positive cells in DIC in the MA group was significantly increased(P<0.05).Immunohistochemistry showed that decidual stromal cells in both groups had CD105 expression in the cytoplasm and nucleus,while the expression level of CD105 in the MA group was significantly higher than that in the control group(P<0.05).Western blot showed that compared with the control group,the expression of CD105 protein in the decidual tissue of the MA group increased(P<0.05).The proportion of MCP-1 positive cells in the two groups was positively correlated with the level of CD105 protein expression(r=0.931 and 0.940,both P<0.01).Conclusion The expression levels of MCP-1 and CD105 in the decidual tissue of MA patients are elevated,which may be associated with the occurrence and development of MA.