斑马鱼癌症模型在肿瘤精准医疗中的研究进展

Research progress of zebrafish patient avatars in precision oncology

在肿瘤精准医疗中,主要采用两种方法来预测临床相关的肿瘤行为(如治疗响应和药物耐药性的出现):基于患者样本基因组、转录组、表观基因组和/或蛋白质组学进行分析推断,以及在个体化癌症患者“替身”中进行表型研究。后一种方法依赖于小鼠体内异种移植模型和体外类器官癌症模型或2D细胞培养。患者源性异种移植免疫受损成年斑马鱼品系的建立,以及基于斑马鱼幼鱼异种移植模型药物反应表型测试的第1项临床试验的开展,使斑马鱼这种小脊椎动物应用于精准医疗领域的前沿。

In precision cancer medicine,two major approaches are used to predict clinically relevant tumor behaviors(such as treatment response and the emergence of drug resistance):analysis and extrapolation based on genome,transcriptome,epigenomics,and/or proteomic of patient samples,and phenotypic studies were performed in individualized models of patients with a cancer.The latter strategy relies on xenograft models in mice and the cancer models of organoids or 2D cell culture in vitro.The establishment of immunocompromised adult zebrafish models of patient-derived xenografts and the development of the first clinical trial based on the drug response test of zebrafish larvae xenografts make this vertebrate to be applied in the frontier of precision medicine.