高效液相色谱法同时测定食品中两种非食用色素的含量

Simultaneous Determination of Two Non-Edible Pigments in Food by HPLC

建立食品中苏丹红Ⅰ、碱性橙残留的高效液相色谱法测定方法,依次用乙腈、甲醇提取样品,合并提取液。采用C_(18)色谱柱、甲醇-乙酸铵流动相进行梯度洗脱,选用紫外检测器450 nm波长进行检测。结果显示,非食用色素苏丹红Ⅰ、碱性橙2、碱性橙21、碱性橙22在各自相应浓度范围内线性相关系数均>0.997;检出限(LOD)0.05 mg·L^(-1),定量限(LOQ)0.20 mg·kg^(-1),线性范围在1.0~10.0 mg·kg^(-1),平均回收率均大于80%,方法稳定可靠,快速准确,重现性好,检测效率高,具有一定的参考价值。

To establish HPLC method for the determination of Sudan red alkaline orange residues in food,the samples were extracted with acetonitrile and methanol successively,and the extracted solution was combined.The detection was performed on a C_(18) column with methanol-ammonium acetate mobile phase,gradient elution,UV detector at 450 nm.The linear correlation coefficients of Sudan red alkaline orange 2 alkaline orange 21 alkaline orange 22 were greater than 0.997 in the corresponding concentration ranges of non-food pigments;the limit of detection(LOD)is 0.05 mg·L^(-1),the limit of quantification(LOQ)is 0.20 mg·kg^(-1),the linear range is 1.0~10.0 mg·kg^(-1),the average recoveries were more than 80%.The method is stable,rapid,accurate,reproducible,high detection efficiency and has a certain reference value.