红茶水提物对小鼠肥胖及肠道微生物的影响

Dose Effects of Black Tea Water Extract on Obesity and Intestinal Microbes in Mice

[目的]研究红茶水提物对小鼠肥胖和肠道微生物的影响。[方法]根据体重相近原则,将实验小鼠分为对照组(CK)、高脂模型组(HFD)、高脂+2.5%红茶水提物(R2.5)、高脂+5%红茶水提物(R5)、高脂+10%红茶水提物(R10)5组,每组6只,喂养56 d。测定小鼠基础指标和血清指标,并通过高通量测序技术研究小鼠肠道微生物菌属相对丰度变化。[结果]不同剂量红茶水提物处理小鼠的体重、体脂率、皮下脂肪率均降低,血清中TG、TC、LDL-C含量降低,且R5处理的效果显著,加大剂量仍能取得相同效果。红茶水提物处理后,小鼠的皮下脂肪率和肝重变化不明显。LefSe分析结果显示,肥胖模型小鼠Akkermansia菌属丰度低于CK,且有害菌Bilophila丰度增加。结合3组小鼠肠道微生物分析,显示Parabacteroide菌属相对丰度随着红茶提取物剂量的上升逐渐下降,Prevotellaceae菌属相对丰度随着红茶水提物剂量的上升而上升。R5组小鼠的Akkermansia菌属相对丰度显著提高,随着红茶水提物剂量的提升,Akkermansia菌属相对丰度没有显著变化。[结论]红茶水提物可以有效缓解小鼠肥胖,能显著影响小鼠肠道中Parabacteroide、Prevotellaceae、Akkermansia的含量。

[Objective]The effect of black tea water extract on obesity and intestinal microbes in mice was studied.[Method]According to the principle of similar body weight,the experimental mice were divided into 5 groups with 6 mice in each group.The five groups were treated separately,followed by the control group(CK),the high-fat model group(HFD),the high-fat+10.5%black tea water extract(R2.5),the high-fat+5.0%black tea water extract(R5),and the high-fat+10.0%black tea water extract(R10),feeding for 8 weeks.Determine the basic indicators and serum indicators of mice,and use high-throughput sequencing technology to study the relative abundance of mouse gut microbes.[Result]The results showed that the body weight,body fat percentage,and subcutaneous fat percentage of mice were reduced after treatment with different doses of black tea water extract,and the serum TG,TC,LDL-C content decreased,and the black tea water extract dose was 5%effective obviously,the same effect can still be obtained by increasing the dose.After the black tea water extract was treated,the subcutaneous fat rate and liver weight of the mice did not change significantly.The results of LefSe analysis showed that the abundance of Akkermansia in obese model mice was lower than that in control mice,and the abundance of harmful bacteria Bilophila increased.Combining the results of the intestinal microbial analysis of the three groups of mice,it was shown that the relative abundance of Parabacteroide gradually decreased with the increase of the black tea extract dosage,and the relative abundance of Prevotellaceae increased with the increase of the black tea water extract dosage.The results showed that the relative abundance of Akkermansia in mice in the R5 group was significantly increased,and the relative abundance of Akkermansia did not change significantly as the dose of black tea water extract increased.[Conclusion]Black tea water extract can effectively relieve obesity,and can significantly affect the contents of Parabacteroide,Prevotellaceae,and Akkermansia in the intestines of mice.