高效液相色谱-二极管阵列法同时测定酱腌菜中5种甜味剂

Simultaneous determination of five sweeteners in pickles by high performance liquid chromatography diode array

建立高效液相色谱-二极管阵列法同时测定酱腌菜中安赛蜜、糖精钠、阿斯巴甜、纽甜、阿力甜的分析检测方法。样品经0.15%甲酸水溶液超声提取、蛋白沉淀、高速离心后,进行梯度洗脱,在25 min内完成分离,外标法定量。试验结果表明,5种甜味剂的检出限为0.6 mg/kg~4.5 mg/kg,回收率在88.5%~103.2%之间。该方法操作简便、快捷,准确可靠,操作性强,可用于酱腌菜中多种甜味剂含量的同时测定。

In order to establish a high performance liquid chromatography-diode array method for simultaneous determination of acesulfect,saccharin sodium,aspartame,neotame and allitame in pickles,the samples were extracted by 0.15%formic acid aqueous solution with ultrasonic extraction,protein precipitation,high-speed centrifugation,gradient elution,separation was completed within 25 min,and quantification was performed by external standard method.The results showed,the detection limits of five sweeteners were 0.6 mg/kg~4.5 mg/kg and the recoveries were 88.5%-103.2%.The method is simple,rapid,accurate and reliable,and can be used for simultaneous determination of various sweeteners in pickles.