茶树CsIQD基因家族的鉴定和表达模式分析

Identification and Expression Pattern Analysis of Cs IQD Gene Family in Camellia sinensis L.

IQD蛋白家族是一类钙调素结合靶蛋白,在植物的生物防御和发育调节中都发挥着重要的作用。为探明茶树中CsIQD基因家族的基本特征,揭示CsIQD基因在茶树中的表达模式,本研究基于茶树基因组数据,利用生物信息学软件对茶树CsIQD家族成员的基因结构、进化关系、保守域及染色体定位等进行分析,并通过Xia等的茶树转录组数据明确了CsIQD在茶树不同组织部位以及PEG诱导的干旱和盐胁迫下的基因表达情况。结果表明,从茶树基因组中共鉴定到38个CsIQD基因,它们在不同的染色体上不均匀地分布排列,按其亲缘关系可分为Ⅳ个蛋白簇,相同亚家族的基因结构和保守结构域相似;分析CsIQD基因在茶树不同组织和胁迫下的表达模式发现,CsIQD基因在芽和嫩叶中有较高的表达,部分基因在花和茎中也有较高表达,在PEG诱导的干旱和盐胁迫下,CsIQD间存在基因的表达差异,因此,CsIQD基因参与了茶树的整个生长过程,同时也是激发茶树非生物胁迫能力的重要调控因子。本研究初步解析了茶树CsIQD基因家族的基本特征和功能,为进一步研究茶树的生长发育调控机制奠定了理论基础。

The IQD protein family is a class of calmodulin binding target proteins,which play an important role in the plant biological defense and developmental regulation.In order to ascertain the basic characteristics of the CsIQD gene family in Camellia sinensis(L.),and reveal the expression pattern of CsIQD genes in Camellia sinensis.This study is based on tea plant genome data,using bioinformatics software to analyze the gene structure,evolutionary relationship,conserved domains,chromosome location,etc.In the members of the CsIQD family,using the transcriptome data of Xia to clnalyze,indicating that CsIQD is in different tissues of Camellia sinensis,and PEG-induced gene expression under the drought and salt stress.The results showed that 38 CsIQD genes were identified from the tea plant genome.They are arranged unevenly on different chromosomes and can be divided intoⅣprotein clusters according to their relationship.The gene structures and conserved domains of the same subfamily are similar;Analyzing the expression patterns of CsIQD gene in different tissues and stresses of tea plants,it is found that CsIQD gene is highly expressed in buds and young leaves,and some genes are also highly expressed in flowers and stems.Under PEG-induced drought and salt stress,there are differences in gene expression between CsIQD.Therefore,the CsIQD gene participates in the entire growth process of tea plants,and is also an important regulatory factor that stimulates the abiotic stress ability of tea plants.This study preliminarily analyzed the basic characteristics and functions of the tea plant CsIQD gene family,and laid a theoretical foundation for further research on the growth and development regulation mechanism of Camellia sinensis.