超高效液相测定茶树水杨酸含量方法的建立

The Establishment of an Ultra-efficient Liquid Phase Method for Determining the Acid Content of Semen in Camellia sinensis

水杨酸的含量对于茶树的生长发育、抗病和抗逆性至关重要。本研究建立了超高效液相色谱提取和检测茶树水杨酸的方法。色谱条件色谱柱 Waters POSSIBLE^(TM) C18 柱(4.6 mm×250 mm,5 h·m);1% 乙酸,1% 磷酸-甲醇(30:70)为流动相;0.8 mL·min^(-1)的流速;30℃的柱温;检测波长276 nm,进样量3h·L。结果表明水杨酸在0.008~0.256 mg·mL^(-1)的浓度范围内线性关系良好(R^(2)=0.999 8),平均回收率为98.13%,相对标准偏差RSD为1.04%。经方法学检验结果表明,该方法精密度高重复性好,满足检测的要求。

The salicylic acid content has been reported to be involved in the plant growth,development,resistance and stress responses.This study established an ultra performance liquid chromatography(UPLC)method for extracting and detecting salicylic acid in tea plants.Chromatography conditions followed by using the waters POSSIBLETM C18 column(4.6 mm×250 mm,5 h·m)as the column;1%acetic acid and 1%phosphoric acid-100% methanol(30:70)as the mobile phase;The flow rate was 0.8 mL·min^(-1);The column temperature was 30℃;detection wavelength was 276 nm;injection volume was 3 h·L.The results showed that the range of salicylic acid concentration from 0.008 to 0.256 mg·mL^(-1) of the paint have a good linear(R^(2)=0.9998),and the average recovery was 98.13%,relative standard deviation(RSD)was 1.04%.The result of the methodology showed that the test method has a high precision and good repeatability,according with the requirement of the detection.